Strawberries (Fragaria × ananassa Duch.) are one of the most economically important fruit crops worldwide, several commercially viable cultivars are cultivated in the northern region of Thailand. The morphological characters at the young vegetative seedling stage can be very similar, which has hindered breeding efforts. The present study assesses the ability of random amplification of polymorphic DNA (RAPD) markers and metabolomics techniques to distinguish six strawberry cultivars. Both techniques showed congruent results for the leaf tissue and classified the cultivars into three major clusters. For the most different cultivars, Akihime and Praratchatan No.80, fruits were analysed at eight fruit ripening stages. The data highlighted a broad biological variation at the early ripening stages and less biological variation at the mature stages. Key metabolic differences included the polyphenol profile in Praratchatan No.80 and fatty acid synthesis/oxidation in Akihime. In summary, the RAPD and metabolite data can be used to distinguish strawberry cultivars and elucidate the metabolite composition of each phenotype. This approach to the characterisation of genotypes will benefit future breeding programmes.

中文翻译：

---

使用 RAPD 标记和代谢物分析技术表征泰国草莓 (Fragaria × ananassa Duch.) 品种

草莓 (Fragaria × ananassa Duch.) 是世界上经济上最重要的水果作物之一，在泰国北部地区种植了几种商业上可行的品种。营养幼苗阶段的形态特征可能非常相似，这阻碍了育种工作。本研究评估了随机扩增多态性 DNA (RAPD) 标记和代谢组学技术以区分六个草莓品种的能力。两种技术在叶组织上都显示出一致的结果，并将栽培品种分为三大类。对于最不同的品种 Akihime 和 Praratchatan No.80，在八个果实成熟阶段对果实进行了分析。数据突出了早熟阶段的广泛生物变异和成熟阶段的较少生物变异。主要代谢差异包括 Praratchatan No.80 中的多酚谱和 Akihime 中的脂肪酸合成/氧化。总之，RAPD和代谢物数据可用于区分草莓品种并阐明每种表型的代谢物组成。这种基因型表征方法将有益于未来的育种计划。