Few reports quantitatively compare the performance of photoacoustic tomography (PAT) versus fluorescence molecular tomography (FMT) in vivo. We compared both modalities for the detection of signals from injected ICG liposomes in the tibial medullary space of 10 BALB/c mice in vivo and ex vivo. Signals significantly correlated between modalities (R² = 0.69) and within each modality in vivo versus ex vivo (PAT: R² = 0.70, FMT: R² = 0.76). Phantom studies showed that signals at 4 mm depth are detected down to 3.3 ng ICG by PAT and 33 ng by FMT, with a nominal spatial resolution below 0.5 mm in PAT and limited to 1 mm in FMT. Our study demonstrates comparable in vivo sensitivity, but superior ex vivo sensitivity and in vivo resolution for our ICG liposomes of the VevoLAZR versus the FMT2500. PAT provides a useful new tool for the high-resolution imaging of bone marrow signals, for example for monitoring drug delivery.

很少有报道定量地比较体内光声层析成像（PAT）与荧光分子层析成像（FMT）的性能。我们比较了两种方式从体内和离体的10只BALB / c小鼠的胫骨髓腔内注射的ICG脂质体的检测信号。信号之间的模式（R 2 = 0.69）之间以及体内与离体（PAT：R 2 = 0.70，FMT：R 2 = 0.76）之间的每种模式之间都具有显着相关性。幻影研究表明，PAT可以检测到4毫米深的信号，ICG可以检测到3.3 ng ICG，FMT可以检测到33 ng的信号，PAT的标称空间分辨率低于0.5 mm，FMT的信号空间分辨率仅限于1 mm。我们的研究表明体内具有可比性VevoLAZR的ICG脂质体相对于FMT2500具有更高的敏感性，但具有更高的离体敏感性和体内分辨率。PAT为骨髓信号的高分辨率成像（例如，监测药物输送）提供了有用的新工具。