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A Novel Plasmid pALWVS1.4 from Acinetobacter lwoffii Strain VS15, Carrying the Chloramphenicol Resistance Gene
Microbiology ( IF 1.5 ) Pub Date : 2020-09-01 , DOI: 10.1134/s0026261720050070
A. Y. Ermakova , A. V. Beletsky , A. V. Mardanov , M. A. Petrova , N. V. Ravin , A. L. Rakitin

Abstract Sequencing and analysis of the genome of the chloramphenicol-resistant strain Acinetobacter lwoffii VS15, isolated from permafrost, revealed a circular plasmid 11 964 bp in length, designated pAL-WVS1.4. Apart from the genes supporting the plasmid maintenance and mobilization, pALWVS1.4 contains the cflA gene encoding the membrane protein of the MFS transporter family. Close homologues of cflA were found in bacteria of the genus Psychrobacter , but were absent in the Acinetobacter genomes. The cflA gene is flanked by two copies of IS elements of the IS 4 family, indicating that it was acquired by A. lwoffii VS15 via horizontal transfer within this putative composite transposon. A recombinant Escherichia coli strain expressing CflA was obtained and it was shown that under conditions of induced expression, the strain was resistant to chloramphenicol.

中文翻译:

一种新型质粒 pALWVS1.4 来自罗氏不动杆菌 VS15 菌株,携带氯霉素抗性基因

摘要 对从永久冻土中分离的耐氯霉素菌株 Acinetobacter lwoffii VS15 的基因组进行测序和分析,揭示了一个长度为 11 964 bp 的环状质粒,命名为 pAL-WVS1.4。除了支持质粒维持和动员的基因外,pALWVS1.4 还包含编码 MFS 转运蛋白家族膜蛋白的 cflA 基因。在 Psychrobacter 属细菌中发现了 cflA 的密切同源物,但在不动杆菌基因组中不存在。cflA 基因的两侧是 IS 4 家族的两个 IS 元件副本,表明它是由 A. lwoffii VS15 通过该假定复合转座子内的水平转移获得的。获得了表达 CflA 的重组大肠杆菌菌株,表明在诱导表达条件下,
更新日期:2020-09-01
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