Abstract The focus of present study was to isolate and characterize toluene metabolizing bacteria and evaluate their bioremediation potential. During present study bacteria were isolated from the tannery effluents using selective enrichment and serial dilution methods. The growth behavior, toluene removal efficiencies, biochemical and molecular characteristics and antibiotic resistance profiles of isolated bacteria were documented through standard procedures. Toluene degradation was also confirmed using GC-MS and LC-MS analysis of metabolites. Present study isolates ( n = 20) showed top BLAST sequence similarity with Brevibacillus agri strain NBRC 15538 (75%), Bacillus paralicheniformis strain KJ-16 (20%) and Burkholderia lata strain 383 (5%). The rRNA gene sequences have been submitted to GeneBank. All present study isolates exhibited remarkable toluene removal potential and most of these were slow-growing. Biochemical characterization indicated bacteria positive for alkaline phosphatases (100%), D-glucosaminidase (35%), naphthylaminopeptidase (15%), esculinase (65%), mannitol (75%), sorbitol (95%) and inulin (90%) fermentation. Biochemical profile suggests the use of isolated bacteria for future exploitation in several fields like toluene bioremediation, ethanol production, biomass hydrolysis, biosensors, biofertilizers, as a marker for milk pasteurization in dairy industries.

中文翻译：

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从制革废水中分离出的多电位甲苯代谢细菌的表征

摘要 本研究的重点是分离和表征甲苯代谢细菌并评估其生物修复潜力。在目前的研究中，使用选择性富集和连续稀释方法从制革废水中分离出细菌。通过标准程序记录分离细菌的生长行为、甲苯去除效率、生化和分子特征以及抗生素抗性特征。使用代谢物的 GC-MS 和 LC-MS 分析也证实了甲苯降解。本研究分离株 (n = 20) 显示与农业短杆菌菌株 NBRC 15538 (75%)、副地衣芽孢杆菌菌株 KJ-16 (20%) 和伯克霍尔德氏菌菌株 383 (5%) 具有最高的 BLAST 序列相似性。rRNA基因序列已提交给GeneBank。所有目前的研究分离株都表现出显着的甲苯去除潜力，其中大部分生长缓慢。生化特征表明细菌对碱性磷酸酶 (100%)、D-氨基葡萄糖苷酶 (35%)、萘胺肽酶 (15%)、七叶苷酶 (65%)、甘露醇 (75%)、山梨糖醇 (95%) 和菊粉 (90%) 呈阳性发酵。生化概况表明，未来在甲苯生物修复、乙醇生产、生物质水解、生物传感器、生物肥料等几个领域中使用分离的细菌作为乳制品行业牛奶巴氏杀菌的标志物。山梨糖醇 (95%) 和菊粉 (90%) 发酵。生化概况表明，未来在甲苯生物修复、乙醇生产、生物质水解、生物传感器、生物肥料等几个领域中使用分离的细菌作为乳制品行业牛奶巴氏杀菌的标志物。山梨糖醇 (95%) 和菊粉 (90%) 发酵。生化概况表明，未来在甲苯生物修复、乙醇生产、生物质水解、生物传感器、生物肥料等几个领域中使用分离的细菌作为乳制品行业牛奶巴氏杀菌的标志物。