Abstract Current study reports isolation of newly isolated slight halophilic bacteria which can utilize 2,4,6-trinitrotoluene (TNT) as a sole nitrogen source, leading to its detoxification. Among 100 halo(alkali)tolerant and halo(alkali)philic strains were isolated from TNT-contaminated soil, two slight halophilic alkalitolerant strains showed remarkable ability to quickly remove the different concentrations of TNT up to 200 mg/L during 24 h in aerobic condition. Phylogenetic analysis indicates that both strains belonged to the genus Pseudomonas which were able to grow aerobically in different concentrations of NaCl (up to 5 and 10%, respectively), temperature (25–40°C), and pH (7–9). Kinetic studies of TNT biotransformation process showed this growth-associated process followed the first-order expression with parameters of k d : 0.09–0.11 mg/(L h), Т 1/2 : 6–7 h, μ: 0.17–0.25 1/h leading to a removal of 90% of TNT (100 mg/L) during a 24 hour period. Finally, the growth rate of both strains evaluated on minimal and complex media, and growth parameters were optimized for maximum growth and most likely maximum biotransformation.

中文翻译：

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新分离的轻嗜盐菌对三硝基甲苯 (TNT) 的生物转化

摘要 目前的研究报告了新分离出的轻度嗜盐细菌的分离，这些细菌可以利用 2,4,6-三硝基甲苯 (TNT) 作为唯一的氮源，导致其解毒。从受 TNT 污染的土壤中分离出的 100 株耐卤（碱）和嗜盐（碱）菌株中，有 2 株轻度嗜盐菌株在有氧条件下，在 24 小时内快速去除高达 200 mg/L 的不同浓度 TNT 的能力显着. 系统发育分析表明，这两种菌株都属于假单胞菌属，它们能够在不同浓度的 NaCl（分别高达 5% 和 10%）、温度（25-40°C）和 pH（7-9）条件下有氧生长。TNT 生物转化过程的动力学研究表明，这种与生长相关的过程遵循 kd 参数的一级表达：0.09–0。11 mg/(L h), Т 1/2 : 6–7 h, μ: 0.17–0.25 1/h 导致在 24 小时内去除 90% 的 TNT (100 mg/L)。最后，在最小和复杂培养基上评估两种菌株的生长速率，并优化生长参数以实现最大生长和最可能的最大生物转化。