The purpose of this study was to produce and characterize Hydroxyapatite/Zinc Oxide/Palladium (HA/0.05 wt% ZnO/0.1 wt% Pd) nanocomposite scaffolds and study their mechanical and antibacterial properties, biocompatibility and bioactivity. The initial materials were developed using sol-gel and precipitation methods. Scaffolds were characterized using atomic absorption analysis (AA), scanning electron microcopy (SEM), energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM), atomic force microscopy (AFM) and Brunauer−EmmeS−Teller (BET) method. Furthermore, the bioactivity of scaffolds in simulated body fluid (SBF) and the interaction of dental pulp stem cells (DPSCs) with the nanocomposite scaffolds were assessed. Our results showed that the HA/ZnO/Pd (H1), HA/ZnO/Pd coated by 0.125 g chitosan (H2) and HA/ZnO/Pd coated by 0.25 g chitosan (H3) scaffolds possess higher compressive strength and toughness and lower microhardness and density compared to the pure HA (H0) scaffolds. Immersion of samples in SBF showed the deposition of apatite on the surface of the scaffolds. The biocompatibility assay indicated lower cell proliferation on the H1, H2 and H3 in comparison to the H0. The antibacterial results obtained show a significant impact by loading Pd/ZnO on HA in the deactivation of microorganisms in vitro.

本研究的目的是生产和表征羟基磷灰石/氧化锌/钯（HA/0.05 wt% ZnO/0.1 wt% Pd）纳米复合支架，并研究它们的机械和抗菌性能、生物相容性和生物活性。最初的材料是使用溶胶-凝胶和沉淀方法开发的。使用原子吸收分析 (AA)、扫描电子显微镜 (SEM)、能量色散光谱 (EDS) 和透射电子显微镜 (TEM)、原子力显微镜 (AFM) 和 Brunauer-EmmeS-Teller (BET) 方法对支架进行表征。此外，还评估了支架在模拟体液 (SBF) 中的生物活性以及牙髓干细胞 (DPSC) 与纳米复合支架的相互作用。我们的结果表明，HA/ZnO/Pd (H1)、0.125 g 壳聚糖 (H2) 包覆的 HA/ZnO/Pd 和 0.125 g 壳聚糖 (H2) 包覆的 HA/ZnO/Pd。与纯 HA (H0) 支架相比，25 g 壳聚糖 (H3) 支架具有更高的抗压强度和韧性以及更低的显微硬度和密度。将样品浸泡在 SBF 中显示磷灰石沉积在支架表面。生物相容性测定表明，与 H0 相比，H1、H2 和 H3 上的细胞增殖较低。获得的抗菌结果表明，将 Pd/ZnO 负载到 HA 上对体外微生物的灭活有显着影响。