In the study, the impact of protonectin (IL-12) on the bioavailability of human lung carcinoma cell line A549 as well as on HFLF healthy lung fibroblast cells was investigated for 24 h and 48 h. Results demonstrated highest cytotoxicity of protonectin on the A549 cell line at 12 µg/ml and 50 µg/ml, and after 48 h and 24 h cell incubation respectively. No cytotoxicity was observed during the incubation of normal HFLF lung cells with the IL-12 peptide. The peptide did not have any significant impact on the hemolysis of human and cattle red blood cells as well as on the viability of human lymphocytes. The effect of protonectin on gene expression of BMI-1 and CD44 as cancer markers was investigated by the real-time RT-PCR. Results indicated that the effect of the peptide on down-regulation of the BMI-1 gene expression was higher compared to CD44 at 12 µg/ml over 24 h and 48 h. A dose and time-dependent pattern was observed in the production of reactive oxygen species (ROS) in the treated A549 cells. From this study, it can be concluded that protonectin has a significant impact on cancer cells for the down-regulation of gene expression of cancer markers and for the up-regulation of the production of ROS.

在这项研究中，研究了24小时和48小时质子蛋白（IL-12）对人肺癌细胞系A549的生物利用度以及对HFLF健康肺成纤维细胞的生物利用度的影响。结果表明，分别在48 µh和24 h细胞孵育后，分别在12 µg / ml和50 µg / ml时，Protonectin对A549细胞株的细胞毒性最高。在正常的HFLF肺细胞与IL-12肽温育期间，未观察到细胞毒性。该肽对人和牛红细胞的溶血以及对人淋巴细胞的活力没有任何显着影响。通过实时逆转录-聚合酶链反应（RT-PCR）研究了质子素对BMI-1和CD44基因表达的影响。结果表明，与CD44相比，在24 h和48 h内，该肽对BMI-1基因表达下调的影响高于CD44（12μg/ ml）。在处理的A549细胞中活性氧（ROS）的产生中观察到剂量和时间依赖性的模式。从这项研究中可以得出结论，对于癌标志物基因表达的下调和ROS产生的上调，质子结合素对癌细胞具有重大影响。