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The counteracting effects of (-)-Epigallocatechin-3-Gallate on the immobilization stress-induced adverse reactions in rat pancreas
Cell Stress and Chaperones ( IF 3.8 ) Pub Date : 2020-10-01 , DOI: 10.1007/s12192-020-01165-2
Nermeen Mohammed Faheem 1, 2 , Tarek Mohamed Ali 3, 4
Affiliation  

Many studies suggest that Epigallocatechin-3-Gallate (EGCG) has many protective effects. But little is known about its protective effects against chronic restraint stress-induced damage in rats. The aim was to demonstrate the potential protective effects of EGCG against harmful pancreatic damage to the immobilization stress in the rat model. Forty rats, 2 months old, were divided into four groups (n = 10): control group; EGCG group, rats received EGCG by gavage (100 mg/kg /day) for 30 days; stressed group, rats exposed to immobilization stress; and stressed with EGCG group, rats exposed to immobilization stress and received EGCG for 30 days. Glycemic status parameters, corticosterone, and inflammatory markers were investigated on the first day, 15th day, and the 30th day of the experiment. Pancreatic oxidative stress markers and cytokines were evaluated. Histological, immunohistological, and statistical studies were performed. On the 15th day, fasting blood glucose (FBG), fasting plasma insulin (FPI), homeostatic model assessment for insulin resistance (HOMA-IR), and fasting plasma corticosterone were significantly higher in the stressed group when compared with first and 30th day in the same group as well as when compared with control and stressed with EGCG groups. The stressed group revealed significantly higher pancreatic IL-1β, IL-6, TNF-α, MDA, and NO, serum amylase and serum lipase, and significantly lower GSH, SOD, and CAT when compared to control and stressed with EGCG groups. EGCG treatment attenuated the pancreatic stress-induced cellular degeneration, leucocytic infiltration, and cytoplasmic vacuolations; significantly decreased area percentage of collagen fibers; and significantly increased mean area percentage of insulin immunopositive cell as compared with stressed group. EGCG is a protective agent against immobilization stress because of its anti-diabetic, anti-inflammatory, and and anti-oxidative stress properties, as confirmed by biochemical and histological alterations.



中文翻译:

(-)-Epigallocatechin-3-Gallate对大鼠胰腺固定化应激不良反应的抑制作用

许多研究表明 Epigallocatechin-3-Gallate (EGCG) 具有许多保护作用。但关于其对大鼠慢性束缚应激损伤的保护作用知之甚少。目的是证明 EGCG 对大鼠模型中固定应激的有害胰腺损伤的潜在保护作用。40 只大鼠,2 个月大,分为四组(n= 10):对照组;EGCG组,大鼠灌胃EGCG(100mg/kg/天)30天;应激组,暴露于固定应激的大鼠;EGCG组应激,大鼠暴露于固定应激并接受EGCG治疗30天。在实验的第一天、第 15 天和第 30 天调查血糖状态参数、皮质酮和炎症标志物。评估了胰腺氧化应激标志物和细胞因子。进行了组织学、免疫组织学和统计研究。第15天,空腹血糖(FBG),空腹血浆胰岛素(FPI),胰岛素抵抗稳态模型评估(HOMA-IR),与同组的第1天和第30天以及与对照组和EGCG组相比,应激组的空腹血浆皮质酮和空腹血浆皮质酮均显着升高。与对照组和 EGCG 组相比,应激组的胰腺 IL-1β、IL-6、TNF-α、MDA 和 NO、血清淀粉酶和血清脂肪酶显着升高,GSH、SOD 和 CAT 显着降低。EGCG 治疗减弱了胰腺应激诱导的细胞变性、白细胞浸润和细胞质空泡化;显着降低胶原纤维的面积百分比;与应激组相比,胰岛素免疫阳性细胞的平均面积百分比显着增加。EGCG 是一种针对固定应激的保护剂,因为它具有抗糖尿病、抗炎、

更新日期:2020-10-02
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