Rocky mountain spotted fever is a severe disease caused by Rickettsia rickettsii that frequently causes the death of the patients. As there are not effective vaccines for this disease, we employed reverse vaccinology to find epitope candidates useful for vaccine development. To apply this bioinformatics, we used the following online software: ProPred1, RANKPEP, and HLA binding, to evaluate 143 amino acid sequences in the genome of Rickettsia rickettsii (NC_009882 Sheila Smith). This strategy allowed us to identify 19 epitope sequences with affinity to HLA I alleles: A0201, A24; HLA-B: B3501, B3901.

中文翻译：

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反向疫苗学在立克氏立克次体的抗原决定簇候选物中的应用

落基山斑热是由立克次氏病引起的一种严重疾病，经常导致患者死亡。由于没有针对该疾病的有效疫苗，我们采用反向疫苗学来寻找可用于疫苗开发的抗原决定簇。要应用此生物信息学，我们使用了以下在线软件：ProPred1，RANKPEP和HLA结合，以评估立克次氏立克次体（NC_009882 Sheila Smith）基因组中的143个氨基酸序列。该策略使我们能够鉴定与HLA I等位基因具有亲和力的19个表位序列：A0201，A24；HLA-B：B3501，B3901。