The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature.

中文翻译：

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在兽医诊断实验室进行实时 PCR 检测的最佳实践

实时聚合酶链反应 (rtPCR) 等体外扩增分析的极高灵敏度需要建立彻底和稳健的实验室实践。为此，美国兽医实验室诊断学家协会 (AAVLD) 的主题专家委员会被召集起来，以制定一套用于核酸扩增检测性能的最佳实践。此处介绍了有关分析前、分析和分析后步骤执行的共识建议，以及对支持文献的回顾。