Abstract Field-evolved resistance to Cry3Bb1 corn by western corn rootworm, Diabrotica virgifera virgifera LeConte (Colleoptera: Chrysomellidae), has been reported in field populations in Iowa, Illinois, Nebraska, and Minnesota. Inheritance and fitness costs associated with Cry3Bb1 resistance have been determined for non-diapausing laboratory strains of western corn rootworm with either laboratory-selected resistance or field-derived resistance. However, information on inheritance and fitness costs of Cry3Bb1 resistance in the diapausing field populations is lacking. In this study, we determined the inheritance of Cry3Bb1 resistance for four diapausing field strains of western corn rootworm using plant-based bioassays. We also determined the fitness costs for eight diapausing field populations in a greenhouse experiment. We found that Cry3Bb1 resistance was an autosomal trait and that the inheritance of resistance was mostly non-recessive; however, there was some variation in the dominance of Cry3Bb1 resistance. We did not find evidence of fitness costs affecting survival to adulthood, developmental rate, or adult dry mass. However, we did detect a fitness cost affecting adult size. The results of this study will add to the current understanding of field-evolved resistance to Cry3Bb1 corn by western corn rootworm and help in developing better strategies to manage resistance.

中文翻译：

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Cry3Bb1抗性在西部玉米根虫（鞘翅目：Chrysomelidae）滞育田间菌株中的遗传和适应成本

摘要 已在爱荷华州、伊利诺伊州、内布拉斯加州和明尼苏达州的田间种群中报道了西部玉米根虫 Diabrotica virgifera virgifera LeConte（鞘翅目：Chrysomellidae）对 Cry3Bb1 玉米的田间进化抗性。对于具有实验室选择抗性或田间抗性的西方玉米根虫的非滞育实验室菌株，已经确定了与 Cry3Bb1 抗性相关的遗传和适应性成本。然而，缺乏关于滞育田间种群中 Cry3Bb1 抗性的遗传和适应成本的信息。在这项研究中，我们使用基于植物的生物测定法确定了四种西方玉米根虫滞育田间菌株的 Cry3Bb1 抗性遗传。我们还确定了温室实验中八个滞育田间种群的适应度成本。我们发现Cry3Bb1抗性是一种常染色体性状，抗性的遗传大多是非隐性的；然而，Cry3Bb1 抗性的优势存在一些差异。我们没有发现健身成本影响成年存活率、发育速度或成人干重的证据。然而，我们确实检测到影响成人体型的健身成本。这项研究的结果将增加目前对西方玉米根虫对 Cry3Bb1 玉米的田间进化抗性的理解，并有助于开发更好的抗性管理策略。我们确实检测到影响成人体型的健身成本。这项研究的结果将增加目前对西方玉米根虫对 Cry3Bb1 玉米的田间进化抗性的理解，并有助于开发更好的抗性管理策略。我们确实检测到影响成人体型的健身成本。这项研究的结果将增加目前对西方玉米根虫对 Cry3Bb1 玉米的田间进化抗性的理解，并有助于开发更好的抗性管理策略。