The aim of our study was to evaluate the influence of low-intensity pulsed US on the delivery of doxorubicin (DOX) into MDA-MB-231 triple-negative breast cancer and A549 non-small cell lung cancer cell 2D and 3D cultures. US with pulse repetition frequency of 10 Hz and 1 MHz center frequency was generated with peak negative pressure of 0.5 MPa and 50% duty cycle. SonoVue microbubbles were used. Spheroids were formed using 3D Bioprinting method. DOX delivery in 2D and 3D cultures was assessed using fluorescence microscopy. US without the addition of microbubbles did not enhance the penetration of DOX into monolayer-cultured cells and tumor spheroids. In the presence of microbubbles US improved the delivery of DOX into the edge end middle zones of A549 and MDA-MB-231 spheroids. Application of low-intensity pulsed US in combination with microbubbles may be a promising approach to enhance the delivery of DOX into tumor spheroids.

我们研究的目的是评估低强度脉冲超声对阿霉素 (DOX) 递送至 MDA-MB-231 三阴性乳腺癌和 A549 非小细胞肺癌细胞 2D 和 3D 培养物的影响。US 脉冲重复频率为 10 Hz，中心频率为 1 MHz，峰值负压为 0.5 MPa，占空比为 50%。使用了 SonoVue 微泡。球体是使用 3D 生物打印方法形成的。使用荧光显微镜评估 2D 和 3D 培养物中的 DOX 递送。不添加微泡的 US 不会增强 DOX 对单层培养细胞和肿瘤球体的渗透。在存在微泡的情况下，美国改善了 DOX 向 A549 和 MDA-MB-231 球体边缘末端中间区域的递送。