The present study is aimed to evaluate the effects of different levels of earthworm (EW) powder and vermihumus (VH) on the growth performance, humoral immunity response, plasma constituents and reproductive performance of Japanese breeder quails. A total of 336 birds were assigned to seven treatments in a completely randomized design from day 30 to 90 of age. The dietary treatments were carried out, one without any addition of EW and VH, the second had a standard rate of VH at 0.8%, while a range of EW additions was supplied from 0%, 0.5%, 1%, 1.5%, 2% and 2.5%. The effects of treatments on body weight and mortality were not significant (p > .05). The highest feed intake was observed in birds fed the diets supplemented with 1% and 1.5% EW (p < .05). The highest titre of antibodies against influenza virus and sheep red blood cells belonged to the EW and VH treatments (p < .05). The antibody titre against Newcastle disease virus vaccine was not affected by the treatments. In general, the birds receiving EW and VH had the highest egg weight, egg production and egg mass weight (p < .05). Nonetheless, various levels of EW powder negatively affected hatchability and increased mortality and leg disorders (p < .05). The results showed that the levels of dietary 1% and 1.5% of EW powder gave the best growth and reproductive performance to birds respectively.

中文翻译：

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含蚯蚓粉和蚯蚓的日粮对日本种鹌鹑产蛋量、孵化率、血液参数和免疫力的影响

本研究旨在评估不同水平的蚯蚓（EW）粉和蚯蚓（VH）对日本种鹌鹑生长性能、体液免疫反应、血浆成分和繁殖性能的影响。从 30 天到 90 天，总共 336 只鸡被分配到完全随机设计的七种处理中。进行了日粮处理，一个不添加任何 EW 和 VH，第二个的 VH 标准率为 0.8%，而 EW 添加范围为 0%、0.5%、1%、1.5%、2 % 和 2.5%。治疗对体重和死亡率的影响不显着（p  > .05）。在饲喂补充 1% 和 1.5% EW 的日粮中观察到的鸡只采食量最高（p < .05）。针对流感病毒和绵羊红细胞的抗体滴度最高的是 EW 和 VH 处理 ( p  < .05)。新城疫病毒疫苗的抗体滴度不受治疗影响。一般来说，接受 EW 和 VH 的鸡只具有最高的蛋重、产蛋量和蛋重（p  < .05）。尽管如此，不同水平的电子战粉末会对孵化率产生负面影响，并增加死亡率和腿部疾病 ( p  < .05)。结果表明，日粮中1%和1.5%的EW粉水平分别为鸟类提供了最佳的生长和繁殖性能。