Interrogation of disease-relevant cellular and molecular traits exhibited by genetically diverse cell populations enables in vitro systems genetics approaches for uncovering the basic properties of cellular function and identity. Primary cells, stem cells, and organoids derived from genetically diverse mouse strains, such as Collaborative Cross and Diversity Outbred populations, offer the opportunity for parallel in vitro/in vivo screening. These panels provide genetic resolution for variant discovery and functional characterization, as well as disease modeling and in vivo validation capabilities. Here we review mouse cellular systems genetics approaches for characterizing the influence of genetic variation on signaling networks and phenotypic diversity, and we discuss approaches for data integration and cross-species validation.

对遗传多样性细胞群表现出的与疾病相关的细胞和分子特征的询问使得体外系统遗传学方法能够揭示细胞功能和身份的基本特性。来自遗传多样性小鼠品系（例如协作杂交和多样性远交群体）的原代细胞、干细胞和类器官为平行体外/体内筛选提供了机会。这些面板为变异发现和功能表征以及疾病建模和体内验证功能提供遗传分辨率。在这里，我们回顾了小鼠细胞系统遗传学方法，用于表征遗传变异对信号网络和表型多样性的影响，并讨论了数据集成和跨物种验证的方法。