The genetic diversity and phylogeny of fast-growing rhizobia isolated from root nodules of Vicia faba grown in different geographical regions of Greece were assessed. Although Rhizobium leguminosarum sv. viciae is the most common symbiont of Vicia spp. in European soils, there is no available information on native rhizobia nodulating faba bean in Greece. Seventy bacterial strains were isolated and grouped into sixteen distinct profiles based on BOX-PCR fingerprinting. The phylogenetic affiliation was further defined by sequence analysis of the rrs and multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and gyrB). Fifty-eight isolates were affiliated with recently described genospecies gsF-2, represented by R. laguerreae FB206^T, whereas six isolates were closely related to gsB and two isolates might belong to gsA. Two isolates assigned to R. hidalgonense and another two non-nodulating strains could not be assigned to any validly defined species and possibly belong to a new rhizobial lineage. Interestingly, R. laguerreae strains were commonly found at all sampling sites, suggesting that they could be the main symbionts of faba beans in Greek soils. According to the phylogenies of two symbiosis-related genes (nodC and nifH), all nodulating isolates belonged to symbiovar (sv.) viciae harboring four distinct nodC gene haplotypes and they were grouped into two clades together with strains assigned to R. laguerreae and genospecies of R. leguminosarum isolated from other countries and continents. This is the first report that R. hidalgonense strains belong to sv. viciae. No correlation was observed between the nodC haplotypes, geographic origin and chromosomal background of the isolates in the study.

评估了从生长在希腊不同地理区域的蚕豆根瘤中分离出来的快速生长的根瘤菌的遗传多样性和系统发育。虽然豆科根瘤菌。蚕豆是蚕豆的最常见共生体。在欧洲土壤中，没有关于希腊根瘤菌蚕豆蚕豆的可用信息。根据BOX-PCR指纹图谱，分离了70个细菌菌株，并分为16个不同的谱。通过rrs的序列分析和三个管家基因（recA，atpD和gyrB）的多位点序列分析（MLSA）进一步定义了系统亲缘关系。）。58个分离物与最近描述的基因组gsF-2关联，由R. laguerreae FB206 ^T代表，而六个分离物与gsB密切相关，两个分离物可能属于gsA。不能将两个分配给hidalgonense的分离株和另外两个非结瘤菌株分配给任何有效定义的物种，并且可能属于新的根瘤菌谱系。有趣的是，在所有采样点都普遍发现拉圭拉氏菌菌株，这表明它们可能是希腊土壤中蚕豆的主要共生体。根据两个与共生相关的基因（nodC和nifH）的系统发育，所有结瘤菌株均属于共生体（sv。）。蚕豆具有四个不同的nodC基因单倍型，它们被分为两个进化枝，以及分配给拉圭雷氏菌的菌株和从其他国家和大洲分离出来的豆科菌的种。这是第一个关于R. hidalgonense菌株属于sv的报道。蚕豆。在研究中，分离株的nodC单倍型，地理起源和染色体背景之间没有相关性。