The highly selective and sensitive fluorometric method has been developed for trace level determination of Hg(II) is based on photo-induced electron transfer between rhodamine-6G dye and metal complex. Quenching in fluorescence intensity by fluorescence resonance energy transfer (FRET) is due to interaction between metal ion complex and dye. The fluorescence emitted was measured at 510 and 550 nm, for excitation and emission wavelengths respectively. Possible interferences present in water samples, which could affect the analytical response are studied and determined. The calibration graph was dynamically linear from 0.002 to 0.05 mgL⁻¹ of Hg(II) with limit of detection 7 × 10⁻⁴ mgL⁻¹ and limit of quantitation 1.9 × 10⁻³ mgL⁻¹. The Stern-Volmer constant (K_SV) calculated for the quenching of R-6G with Hg (II) was 8.47 Lmg⁻¹ s⁻¹ at optimized reaction conditions. The proposed FRET based fluorometric method was applied successfully in different industrial wastewater samples with satisfactory outcome.

已经开发出了一种高选择性和灵敏的荧光法，用于基于痕量罗丹明6G染料和金属配合物的光诱导电子转移来测定痕量汞（II）。由于金属离子络合物和染料之间的相互作用，通过荧光共振能量转移（FRET）导致的荧光强度猝灭。在激发和发射波长分别在510和550 nm处测量发射的荧光。研究并确定了水样品中可能影响分析响应的可能干扰。校准曲线从0.002到0.05 mgL ^-1的Hg（II）是线性的，检出限为7×10 ^-4  mgL ^-1，定量限为1.9×10 ^-3  mgL^-1。在优化的反应条件下，用Hg（II）淬灭R-6G的Stern-Volmer常数（K _SV）为8.47 Lmg ^-1  s ^-1。所提出的基于FRET的荧光法已成功应用于不同的工业废水样品中，并获得满意的结果。