Background

Mesenchymal stem cells (MSCs) in synovial fluid increase after traumatic meniscus injuries. However, MSC kinetics in synovial fluid may differ for knees with degenerative meniscus injuries. Furthermore, the combination of surgical repair and synovial MSC transplantation has been found to improve clinical symptoms in patients with degenerative meniscus injury, and in this treatment, only the operation procedure without MSC transplantation might increase MSCs in synovial fluid; if so, soluble factors in synovial fluid will be involved. The purpose is this study was to examine whether MSCs exist in synovial fluid of knees with degenerative meniscus injury, to investigate whether MSCs in synovial fluid increase after harvest of synovium and meniscus repair, and to explore what soluble factors in synovial fluids affect the number of MSCs in synovial fluid.

Methods

Subjects were 7 patients with degenerative meniscus injury who underwent meniscal repair and synovial MSC transplantation. Synovial fluid (Pre) was aspirated from knees before harvest of synovium and meniscus repair. After 2 weeks, synovial fluid (Post) was aspirated again before transplantation of synovial MSCs. A half volume of the synovial fluid was plated and cultured for 2 weeks to count the colony formation. The other half was used for antibody array analysis, and the correlation coefficients between the signal intensity and colony number were measured in 503 factors. Factors with high correlation coefficients were verified by migration assay.

Results

While cell colonies derived from synovial fluid (Pre) were hardly observed, greater numbers of colonies from synovial fluid (Post) were demonstrated. Of the 503 factors, calcitonin gene-related peptide (CGRP) and hepatocyte growth factor (HGF) had high correlation coefficients between colony number and expression level. Both CGRP and HGF promoted migration of synovial fluid MSCs.

Conclusions

MSCs in synovial fluid were hardly seen in knees with degenerated meniscus injury. They significantly increased 2 weeks after harvest of synovium and meniscus repair. Both CGRP and HGF in synovial fluid can possibly induce MSCs from synovium into synovial fluid.

中文翻译：

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关节镜手术后退行性半月板损伤膝关节滑液中的间充质干细胞通过 CGRP 和 HGF 的内源性作用增加

背景

创伤性半月板损伤后滑液中的间充质干细胞 (MSCs) 增加。然而，对于退行性半月板损伤的膝盖，滑液中的 MSC 动力学可能不同。此外，已经发现手术修复和滑膜间充质干细胞移植的结合可以改善退行性半月板损伤患者的临床症状，并且在这种治疗中，只有没有进行间充质干细胞移植的手术程序可能会增加滑液中的间充质干细胞；如果是这样，将涉及滑液中的可溶性因子。本研究的目的是检测退行性半月板损伤膝关节滑液中是否存在间充质干细胞，研究滑液收获和半月板修复后滑液中的间充质干细胞是否增加，

方法

受试者为 7 名接受半月板修复和滑膜 MSC 移植的退行性半月板损伤患者。在收获滑膜和半月板修复之前，从膝盖中吸出滑液 (Pre)。2周后，再次抽吸滑液（Post），然后移植滑膜间充质干细胞。将一半体积的滑液接种并培养 2 周以计数集落形成。另一半用于抗体阵列分析，在503个因子中测量信号强度与菌落数的相关系数。通过迁移测定验证具有高相关系数的因素。

结果

虽然几乎没有观察到来自滑液 (Pre) 的细胞集落，但证明了来自滑液 (Post) 的更多数量的集落。在503个因子中，降钙素基因相关肽（CGRP）和肝细胞生长因子（HGF）在集落数和表达水平之间具有较高的相关系数。CGRP 和 HGF 均促进滑液 MSC 的迁移。

结论

在退行性半月板损伤的膝盖中几乎看不到滑液中的间充质干细胞。它们在收获滑膜和半月板修复后 2 周显着增加。滑液中的CGRP和HGF都可能诱导MSC从滑膜进入滑液。