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Finding new addictive QTL for yield traits based on a high-density genetic map in hybrid rice
Plant Growth Regulation ( IF 4.2 ) Pub Date : 2020-09-30 , DOI: 10.1007/s10725-020-00669-2
Miao Zhang , Zheng-ping Zhou , Yu-yu Chen , Yong-run Cao , Chen-wei Deng , Pao Xue , Xiao-deng Zhan , Shi-hua Cheng , Li-yong Cao , Ying-xin Zhang

Rice is one of the most important food crops in the world. To discover the genetic basis of yield components in super hybrid rice Nei2You No.6, 386 recombinant inbred sister lines (RISLs) were obtained for mapping quantitative trait loci (QTL) responsible for grain yield per plant, number of panicles per plant, grain number per panicle and 1000-grain weight. Using whole genome re-sequencing, a high-density linkage map consisting of 3203 Bin markers was constructed with total genetic coverage of 1951.1 cM and average density of 0.61 cM. A total of 43 yield-related QTL were mapped to all 12 chromosomes, and each explained 2.40–10.17% phenotypic variance, indicating that the medium and minor effect QTL are genetic basis for high yield of Nei2You No.6. With positive effect, 28 out of the 43 QTL are inherited from the maintainer line (Nei2B). Nine loci, qGYP-6b , qGNP-6c , qNP-7 , qTGW-1a , qTGW-5 , qTGW-7 , qTGW-10b , qTGW-10c and qTGW-12 showed stable effects across multiple environments. Five of these nine QTL were co-located with previously reported QTL, and four novel locus, qNP-7 , qGNP-6c , qTGW-7 and qTGW-12 , were firstly identified in the present study. Subsequently, qNP-7 , qTGW-7 and qTGW-12 were validated using corresponding paired sister lines which differed only in the target genome region. The recombinant inbred sister lines is an effective tool for mapping and confirming QTL of yield-associated traits. Newly detected QTL provide new resource for investigating genetics of yield components and will accelerate molecular breeding in rice.

中文翻译:

基于杂交水稻高密度遗传图谱寻找新的产量性状上瘾QTL

水稻是世界上最重要的粮食作物之一。为发现超级杂交稻内2优6号产量成分的遗传基础,获得了386个重组自交姐妹系(RISL),用于定位影响单株产量、单株穗数、粒数的数量性状位点(QTL)。每穗和 1000 粒重。使用全基因组重测序,构建了由3203个Bin标记组成的高密度连锁图,总遗传覆盖率为1951.1 cM,平均密度为0.61 cM。共有43个产量相关QTL定位到全部12条染色体,每个QTL解释了2.40-10.17%的表型变异,表明中效和微效QTL是内2优6号高产的遗传基础。具有积极作用的是,43 个 QTL 中有 28 个继承自保持系 (Nei2B)。九处,qGYP-6b、qGNP-6c、qNP-7、qTGW-1a、qTGW-5、qTGW-7、qTGW-10b、qTGW-10c和qTGW-12在多种环境中表现出稳定的效果。这九个 QTL 中有五个与先前报道的 QTL 位于同一位置,本研究首次鉴定了四个新位点 qNP-7、qGNP-6c、qTGW-7 和 qTGW-12。随后,qNP-7、qTGW-7 和 qTGW-12 使用相应的配对姐妹系进行验证,这些姐妹系仅在目标基因组区域有所不同。重组自交姐妹系是绘制和确认产量相关性状 QTL 的有效工具。新发现的QTL为研究产量成分的遗传学提供了新的资源,将加速水稻的分子育种。这九个 QTL 中有五个与先前报道的 QTL 位于同一位置,本研究首次鉴定了四个新位点 qNP-7、qGNP-6c、qTGW-7 和 qTGW-12。随后,qNP-7、qTGW-7 和 qTGW-12 使用相应的配对姐妹系进行验证,这些姐妹系仅在目标基因组区域有所不同。重组自交姐妹系是绘制和确认产量相关性状 QTL 的有效工具。新发现的QTL为研究产量成分的遗传学提供了新的资源,将加速水稻的分子育种。这九个 QTL 中有五个与先前报道的 QTL 位于同一位置,本研究首次鉴定了四个新位点 qNP-7、qGNP-6c、qTGW-7 和 qTGW-12。随后,qNP-7、qTGW-7 和 qTGW-12 使用相应的配对姐妹系进行验证,这些姐妹系仅在目标基因组区域有所不同。重组自交姐妹系是绘制和确认产量相关性状 QTL 的有效工具。新发现的QTL为研究产量成分的遗传学提供了新的资源,将加速水稻的分子育种。qTGW-7 和 qTGW-12 使用相应的配对姐妹系进行验证,这些姐妹系仅在目标基因组区域上有所不同。重组自交姐妹系是绘制和确认产量相关性状 QTL 的有效工具。新发现的QTL为研究产量成分的遗传学提供了新的资源,将加速水稻的分子育种。qTGW-7 和 qTGW-12 使用相应的配对姐妹系进行验证,这些姐妹系仅在目标基因组区域有所不同。重组自交姐妹系是绘制和确认产量相关性状 QTL 的有效工具。新发现的QTL为研究产量成分的遗传学提供了新的资源,将加速水稻的分子育种。
更新日期:2020-09-30
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