A growing number of studies suggest that plant viruses manipulate host plant phenotypes to increase transmission-conducive behaviors by vectors. Studies on this phenomenon frequently omit examination of interactions that occur after vectors acquire virions, which provides an incomplete understanding of the ecology of plant virus manipulation. Here, by taking a full factorial approach that considered both the infection status of the host (Montia perfoliata) and viruliferous status of the aphid (Myzus persicae), we explored the effects of a circulative, non-propagative virus (Turnip yellows virus [TuYV]) on a suite of behavior and performance metrics that are relevant for virus transmission. Our results demonstrate that viruliferous aphids exhibited an increased velocity of movement and increased activity levels in locomotor and dispersal-retention assays. They also had increased fecundity and showed a capacity to more efficiently exploit resources by taking less time to reach the phloem and ingesting more sap, regardless of plant infection status. In contrast, non-viruliferous aphids only exhibited enhanced fecundity and biomass on TuYV-infected hosts, and had overall reduced dispersal and locomotor activity relative to viruliferous aphids. In this pathosystem, post-acquisition effects were stronger and more conducive to virus transmission than the purely pre-acquisition effects mediated by virus effects on the host plant. Our study provides additional support for the hypothesis that virus manipulation of vector behavior includes both pre- and post-acquisition effects and demonstrates the importance of considering both components when studying putative virus manipulation strategies.

越来越多的研究表明，植物病毒操纵宿主植物的表型，以增加载体的传播传导行为。对这种现象的研究经常省略对载体获取病毒体后发生的相互作用的检查，这使人们对植物病毒操纵的生态学缺乏完全的了解。在这里，通过考虑宿主的感染状态（Montia perfoliata）和蚜虫的有毒状态（Myzus persicae）的完全因子分析方法，我们探索了循环非传播病毒（芜菁黄病毒）的影响[TuYV]）一系列与病毒传播相关的行为和性能指标。我们的结果表明，在运动和扩散保留分析中，有毒的蚜虫表现出更高的运动速度和更高的活性水平。他们还增加了繁殖力，并显示出通过花费更少的时间到达韧皮部和摄入更多汁液而更有效地利用资源的能力，而与植物的感染状况无关。相比之下，非毒力蚜虫仅在感染TuYV的宿主上表现出增强的繁殖力和生物量，并且相对于毒力蚜虫总体上降低了传播和运动能力。在这种病理系统中，采集后的作用比病毒对宿主植物介导的纯粹的采集前作用更强，更有利于病毒传播。