Sub-Antarctic macroalgae can be a feasible source of innumerous bioactive compounds that can display important biological activities. In this sense, the aims of this work were to evaluate the chemical composition, antimicrobial activity and cytotoxic potential of n-hexane extracts from sub-Antarctic seaweed Mazzaella laminarioides (Bory) Fredericq at distinct development phases. Results showed that extracts were constituted of as much as 65 distinct compounds that could be distributed mainly among hydrocarbons, fatty acids, sterols and fatty alcohols. Generally, there were significant differences (P < 0.05) in the concentrations of compounds comparing distinct development phases. Antimicrobial evaluation indicated that n-hexane extracts of cystocarpic and tetrasporophytic phase of M. laminarioides inhibited the growth of most organisms tested, reaching minimum inhibitory concentrations of 0.0312 mg mL−1 against Enterococcus faecalis (Andrewes and Horder) and 0.25 mg mL−1 against Acinetobacter baumannii (Bouvet and Grimont). Cytotoxic evaluation of extracts showed no toxicity to normal cells at concentrations below 1 mg mL−1. Therefore, M. laminarioides from the sub-Antarctic region had antimicrobial potential that could be explored on further commercial applications.

中文翻译：

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不同发育阶段亚南极大型藻类海藻提取物的生物活性评价和组成

亚南极大型藻类可以成为无数具有重要生物活性的生物活性化合物的可行来源。从这个意义上说，这项工作的目的是评估来自亚南极海藻 Mazzaella laminarioides (Bory) Fredericq 的正己烷提取物在不同发育阶段的化学成分、抗菌活性和细胞毒性潜力。结果表明，提取物由多达 65 种不同的化合物组成，这些化合物主要分布在碳氢化合物、脂肪酸、甾醇和脂肪醇中。一般来说，比较不同发展阶段的化合物浓度存在显着差异（P < 0.05）。抗菌评估表明，海带丝藻的囊果和四孢子相的正己烷提取物抑制了大多数测试生物的生长，对粪肠球菌（Andrewes 和 Horder）的最低抑制浓度为 0.0312 mg mL-1，对鲍曼不动杆菌（Bouvet 和 Grimont）的最低抑制浓度为 0.25 mg mL-1。提取物的细胞毒性评估表明，浓度低于 1 mg mL-1 时对正常细胞没有毒性。因此，来自亚南极地区的 M. laminarioides 具有抗菌潜力，可以在进一步的商业应用中进行探索。