The “alperujo” is a waste from the olive oil industry with great potential for valorization. It has a high organic load, with the presence of valuable compounds such as biophenols and sugars. The use of this waste can be thought of as a biorefinery from which different compounds of high added value can be obtained, whether they are present in the “alperujo” such as biophenols or can be generated from the “alperujo”. Therefore, the production of carotenoids by Rhodotorula mucilaginosa was evaluated using the liquid fraction of ‘alperujo’ (Alperujo Water, AW) or an aqueous extract (AE) of “alperujo” at different concentrations (5, 10, 20 and 30% w/V) as substrates. The AEs had an acidic pH, a total sugar concentration ranging from 1.6 to 7.6 g/L, a polyphenols content from 0.4 to 2.9 g/L and a significant amount of proteins (0.5–3 g/L). AW is similar in composition as 30% AE, but with a higher amount of total sugars. Rh. mucilaginosa was able to grow at the different mediums with consumption of glucose and fructose, a reduction in protein content and alkalinization of the medium. Maximum total carotenoid production (7.3 ± 0.6 mg/L) was achieved at AW, while the specific production was higher when the yeast grew at AW or at 30% AE (0.78 ± 0.06 and 0.73 ± 0.10 mg/g of biomass, respectively). Torulene and torularhodin were the main carotenoids produced. Polyphenol content did not change; thus, it is still possible to recover these compounds after producing carotenoids. These results demonstrate the feasibility of using alperujo-based mediums as cheap substrates to produce torularhodin and torulene and to include this bioprocess as a step in an integral approach for alperujo valorization.

“ alperujo”是橄榄油行业的一种废物，具有很大的增值潜力。它具有很高的有机负荷，并存在有价值的化合物，例如生物酚和糖。可以将这种废物的用途视为一种生物精炼厂，从中可以获得高附加值的不同化合物，无论它们存在于“ alperujo”中，例如生物酚还是可以从“ alperujo”中产生。因此，粘液红假单胞菌产生类胡萝卜素使用“ alperujo”的液体部分（Alperujo Water，AW）或“ alperujo”的水提取物（AE）以不同浓度（5、10、20和30％w / V）作为底物进行评估。AE具有酸性pH值，总糖浓度范围为1.6至7.6 g / L，多酚含量为0.4至2.9 g / L和大量蛋白质（0.5–3 g / L）。AW的成分类似于AE的30％，但总糖含量较高。铑 粘菌皮能够消耗葡萄糖和果糖，减少蛋白质含量，使培养基碱化，从而在不同的培养基上生长。AW达到最大的类胡萝卜素总产量（7.3±0.6 mg / L），而酵母以AW或30％AE生长时的比产量更高（分别为0.78±0.06和0.73±0.10 mg / g生物量） 。产生的主要类胡萝卜素是Torulene和torularhodin。多酚含量没有变化；因此，在生产类胡萝卜素后仍然有可能回收这些化合物。这些结果证明了使用基于alperujo的培养基作为便宜的底物来生产torularhodin和torulene以及将该生物过程包括为alperujo增值的整体方法中的一个步骤的可行性。