The aim of the present study was to generate a consistent data set for biorefinery intermediates from novel energy crop Silphium perfoliatum compared with Fagus sylvatica, including process streams from liquid hot water pretreatment, enzymatic hydrolysis, and fermentation with Bacillus licheniformis. The consistency of the data set was further supported by the application of a consistent analytical method based on high performance anion exchange chromatography with pulsed amperometric detection and validated for process intermediates, which renders the technique a versatile analytical tool also for alcoholic compounds. For the first time, Silphium perfoliatum was used for liquid hot water pretreatment which resulted in a maximal absolute glucose recovery after enzymatic hydrolysis for feedstock pretreated with 200 °C, 20 min, 20% solid loading. Under these conditions, 68% glucose were recovered for Silphium perfoliatum and 62% for Fagus sylvatica. Further, enzymatic hydrolyzates of both feedstocks were successfully used as single carbon sources for 2,3-butanediol fermentation with Bacillus licheniformis resulting in a 2,3-butanediol yield of 39% and 31% of the theoretical yield for Silphium perfoliatum and Fagus sylvatica, respectively. Thus, the technical suitability of Silphium perfoliatum as feedstock for a liquid hot water–based biorefinery process was comprehensively demonstrated and successfully bore the comparison with Fagus sylvatica.

本研究的目的是为新型能源作物过氧化硅与水青冈（Fagus sylvatica）生成生物炼制中间体的一致数据集，包括液体热水预处理，酶促水解和地衣芽孢杆菌发酵的工艺流。数据集的一致性还通过应用基于高效阴离子交换色谱和脉冲安培检测的一致分析方法的应用得到了进一步的支持，并经过了工艺中间体的验证，这使该技术也成为了一种用于醇类化合物的通用分析工具。第一次，Silphium perfoliatum将其用于液体热水预处理，酶水解后，经200°C，20分钟，20％固体负载预处理的原料可实现最大的绝对葡萄糖回收率。在这些条件下，过氧化硅回收了68％的葡萄糖，而青冈则回收了62％的葡萄糖。此外，两种原料的酶解产物已成功用作地衣芽孢杆菌2,3丁二醇发酵的单一碳源，导致2,3丁二醇的收率分别为生叶紫苏和Fagus sylvatica的39％和理论收率的31％，分别。因此，Silphium perfoliatum的技术适用性作为基于液态热水的生物精炼过程的原料，已得到全面证明，并成功地与西格斯（Fagus sylvatica）进行了比较。