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In vitro culture method of Ditylenchus destructor (Tylenchida: Anguinidae) using garlic
Applied Entomology and Zoology ( IF 1.3 ) Pub Date : 2020-09-29 , DOI: 10.1007/s13355-020-00701-x
Toyoshi Yoshiga

Ditylenchus destructor Thorne (Tylenchida: Anguinidae) is a plant-parasitic nematode that causes garlic rot in Japan. A method that can be used to culture the nematode on garlic has not yet been established, but is required to be able to conduct studies for a better understanding of the nematode’s physiology and ecology. The purpose of this study was to develop such a culture method. Surface-sterilized nematodes (200 individuals) were inoculated onto a surface-sterilized garlic storage leaf on 1% agar in a 100 ml medium bottle, and incubated at 25 °C. Seven weeks after inoculation, a mean number of approximately 360,000 axenic nematodes per bottle was obtained. The culture method established in this study is simple, less laborious, and can be useful for the studies of D. destructor associated with garlic.

中文翻译:

使用大蒜的Ditylenchus destructor (Tylenchida: Anguinidae) 体外培养方法

Ditylenchus destructor Thorne (Tylenchida: Anguinidae) 是一种在日本引起大蒜腐烂的植物寄生线虫。可用于在大蒜上培养线虫的方法尚未建立,但需要能够进行研究以更好地了解线虫的生理和生态。本研究的目的是开发这样一种培养方法。将表面灭菌的线虫(200 只)接种到表面灭菌的大蒜贮藏叶上,置于 100 ml 培养基瓶中的 1% 琼脂上,并在 25°C 下孵育。接种七周后,每瓶获得平均约 360,000 条无菌线虫。本研究建立的培养方法简单、省力,可用于与大蒜相关的 D. destructor 的研究。
更新日期:2020-09-29
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