Three new coordination polymers (CPs), namely [{M(HL)(L)(H₂O)}(ClO₄)(H₂O)]_∞ (M = Zn for CP 1, Mn for CP 2, Cu for CP 3) were synthesized to explore their efficacy as lysosome-targetable luminescent bioprobes. The synthesized CPs were characterized by techniques including single-crystal X-ray analysis, FTIR spectroscopy and elemental analysis. Single-crystal analysis revealed the formation of iso-structural CPs displaying distorted adamantoid topology developed by bridging ligands and H-bonds connections and metals at the nodes. A green hand-grinding technique with a mortar and pestle resulted in nanoscale coordination polymers (NCPs) suitable for cell permeability and was further confirmed by SEM and DLS analyses. Two of these hand-ground nanoscale coordination polymers NCP 1 and NCP 2 showed excellent green luminescence and were explored as potential and selective long-time biotrackers towards lysosome using the human lung carcinoma cell line (A549). Strikingly, the developed bioprobe displayed excellent bio-availability, photostability and excellent selectivity towards lysosomes sustained by various in vitro cell imaging experiments. Moreover, the long-term probing ability of these NCPs turned out to be better than the commercially available lysosome tracker i.e. LysoTracker Red, indicating their potential real-life application in bio-imaging. To the best ofour knowledge, this is the first example of nonexpensive and less toxic essential transition metal-based nanoscale coordination polymers that can behave as effective lysosome-targetable luminescent bioprobes.

中文翻译：

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绿色便利的合成方法可开发可作为溶酶体靶向的发光生物探针的纳米级配位聚合物

三个新的配位聚合物（CP或多个），即[{M（HL）（L）（H ₂ O）}（CLO ₄）（H ₂ O）] _∞（M =锌为CP 1，锰为CP 2，铜对于CP 3）的合成探索它们的功效如溶酶体靶向发光生物探针。合成的CP通过单晶X射线分析，FTIR光谱和元素分析等技术进行表征。单晶分析表明同构CP的形成s显示了扭曲的金刚烷拓扑，该拓扑是通过在节点处桥接配体和氢键连接以及金属而形成的。用研钵和杵进行的绿色手工研磨技术产生了适合细胞渗透性的纳米级配位聚合物（NCP），并通过SEM和DLS分析得到了进一步证实。这些手工研磨的纳米级配位聚合物NCP 1和NCP 2中的两种显示出优异的绿色发光，并被人类肺癌细胞系（A549）用作溶酶体的潜在和选择性长期生物示踪剂。令人惊讶的是，开发的生物探针显示出优异的生物利用度，光稳定性和对各种体外维持的溶酶体的优异选择性。细胞成像实验。此外，事实证明，这些NCP的长期探测能力要优于市售的溶酶体示踪剂，即LysoTracker Red，表明它们在生物成像中的潜在现实应用。据我们所知，这是无价且毒性较小的必需过渡金属基纳米级配位聚合物的第一个实例，该聚合物可充当有效的溶酶体靶向发光生物探针。