Serine/threonine protein kinase-3 (STK3) is a critical molecule of the Hippo pathway but little is known about its biological functions in the ovarian cancer development. We demonstrated the roles of STK3 in ovarian cancer. Existing databases were used to study the expression profile of STK3. STK3 was significantly downregulated in OC patients, and the low STK3 expression was correlated with a poor prognosis. In vitro cell proliferation, apoptosis, and migration assays, and in vivo subcutaneous xenograft tumor models were used to determine the roles of STK3. The overexpression of STK3 significantly inhibited cell proliferation, apoptosis, and migration of ovarian cancer cells in vitro and tumor growth in vivo. Bisulfite sequencing PCR analysis was performed to validate the methylation of STK3 in ovarian cancer. RNA sequencing and gene set enrichment analysis (GSEA) were used to compare the transcriptome changes in the STK3 overexpression ovarian cancer and control cells. The signaling pathway was analyzed by western blotting. STK3 promoted the migration of CD8⁺ T-cells by activating nuclear transcription factor κB (NF-κB) signaling. STK3 is a potential predictor of OC. It plays an important role in suppressing tumor growth of ovarian cancer and in chemotaxis of CD8⁺ T-cells.

中文翻译：

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STK3通过激活NF-κB信号传导招募CD8 + T细胞来抑制卵巢癌进展。

丝氨酸/苏氨酸蛋白激酶3（STK3）是Hippo通路的关键分子，但其在卵巢癌发展中的生物学功能知之甚少。我们证明了STK3在卵巢癌中的作用。现有数据库用于研究STK3的表达谱。OC患者中STK3明显下调，STK3表达低与预后不良有关。体外细胞增殖，凋亡和迁移测定以及体内皮下异种移植肿瘤模型被用于确定STK3的作用。STK3的过表达在体外和体内显着抑制卵巢癌细胞的细胞增殖，凋亡和迁移。进行亚硫酸氢盐测序PCR分析以验证STK3在卵巢癌中的甲基化。RNA测序和基因集富集分析（GSEA）用于比较STK3过表达卵巢癌和对照细胞中转录组的变化。通过蛋白质印迹分析信号传导途径。STK3促进了CD8的迁移⁺ T细胞通过激活核转录因子κ B（NF- κ B）的信令。STK3是OC的潜在预测因子。它在抑制卵巢癌的肿瘤生长和CD8 ⁺ T细胞的趋化性中起重要作用。