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Breathomics in Asthmatic Children Treated with Inhaled Corticosteroids
Metabolites ( IF 4.1 ) Pub Date : 2020-09-29 , DOI: 10.3390/metabo10100390 Valentina Agnese Ferraro , Silvia Carraro , Paola Pirillo , Antonina Gucciardi , Gabriele Poloniato , Matteo Stocchero , Giuseppe Giordano , Stefania Zanconato , Eugenio Baraldi
Metabolites ( IF 4.1 ) Pub Date : 2020-09-29 , DOI: 10.3390/metabo10100390 Valentina Agnese Ferraro , Silvia Carraro , Paola Pirillo , Antonina Gucciardi , Gabriele Poloniato , Matteo Stocchero , Giuseppe Giordano , Stefania Zanconato , Eugenio Baraldi
Background: “breathomics” enables indirect analysis of metabolic patterns underlying a respiratory disease. In this study, we analyze exhaled breath condensate (EBC) in asthmatic children before (T0) and after (T1) a three-week course of inhaled beclomethasone dipropionate (BDP). Methods: we recruited steroid-naive asthmatic children for whom inhaled steroids were indicated and healthy children, evaluating asthma control, spirometry and EBC (in asthmatics at T0 and T1). A liquid-chromatography–mass-spectrometry untargeted analysis was applied to EBC and a mass spectrometry-based target analysis to urine samples. Results: metabolomic analysis discriminated asthmatic (n = 26) from healthy children (n = 16) at T0 and T1, discovering 108 and 65 features relevant for the discrimination, respectively. Searching metabolomics databases, seven putative biomarkers with a plausible role in asthma biochemical–metabolic processes were found. After BDP treatment, asthmatic children, in the face of an improved asthma control (p < 0.001) and lung function (p = 0.01), showed neither changes in EBC metabolomic profile nor in urinary endogenous steroid profile. Conclusions: “breathomics” can discriminate asthmatic from healthy children, with prostaglandin, fatty acid and glycerophospholipid as putative markers. The three-week course of BDP—in spite of a significant clinical improvement—was not associated with changes in EBC metabolic arrangement and urinary steroid profile.
中文翻译:
吸入性糖皮质激素治疗哮喘儿童的呼吸动力学
背景:“同性恋”可以间接分析呼吸系统疾病的潜在代谢模式。在这项研究中,我们分析了哮喘儿童吸入三倍疗程的倍氯米松二丙酸酯(BDP)之前(T0)和之后(T1)的呼出气冷凝物(EBC)。方法:我们招募了未接受类固醇吸入治疗的类固醇哮喘儿童和健康儿童,评估了哮喘控制,肺活量测定和EBC(在T0和T1的哮喘患者中)。液相色谱-质谱无目标分析应用于EBC,而基于质谱的目标分析应用于尿液样品。结果:代谢组学分析将哮喘(n = 26)与健康儿童(n= 16)在T0和T1处,分别发现与辨别相关的108和65个特征。在代谢组学数据库中,发现了7种在哮喘生化代谢过程中可能起作用的生物标记。BDP治疗后,哮喘儿童面对改善的哮喘控制(p <0.001)和肺功能(p = 0.01),既未显示EBC代谢组学特征也未显示尿内源性类固醇激素变化。结论:“烈酒”可以区分健康儿童与哮喘儿童,前列腺素,脂肪酸和甘油磷脂是公认的标志物。尽管临床上已有显着改善,但BDP的三周疗程与EBC代谢安排和尿类固醇激素谱的变化无关。
更新日期:2020-09-29
中文翻译:
吸入性糖皮质激素治疗哮喘儿童的呼吸动力学
背景:“同性恋”可以间接分析呼吸系统疾病的潜在代谢模式。在这项研究中,我们分析了哮喘儿童吸入三倍疗程的倍氯米松二丙酸酯(BDP)之前(T0)和之后(T1)的呼出气冷凝物(EBC)。方法:我们招募了未接受类固醇吸入治疗的类固醇哮喘儿童和健康儿童,评估了哮喘控制,肺活量测定和EBC(在T0和T1的哮喘患者中)。液相色谱-质谱无目标分析应用于EBC,而基于质谱的目标分析应用于尿液样品。结果:代谢组学分析将哮喘(n = 26)与健康儿童(n= 16)在T0和T1处,分别发现与辨别相关的108和65个特征。在代谢组学数据库中,发现了7种在哮喘生化代谢过程中可能起作用的生物标记。BDP治疗后,哮喘儿童面对改善的哮喘控制(p <0.001)和肺功能(p = 0.01),既未显示EBC代谢组学特征也未显示尿内源性类固醇激素变化。结论:“烈酒”可以区分健康儿童与哮喘儿童,前列腺素,脂肪酸和甘油磷脂是公认的标志物。尽管临床上已有显着改善,但BDP的三周疗程与EBC代谢安排和尿类固醇激素谱的变化无关。