Methicillin-resistant Staphylococcus aureus (MRSA) harboring the type-IX staphylococcal cassette chromosome mec (SCCmec) has been found in pigs and humans in Northern Thailand. However, knowledge of the prevalence and acquisition risk factors of this MRSA strain among swine production personnel (SPP) are needed. The nasal swab samples and data were collected from 202 voluntary SPP and 31 swine farms in Chiang Mai and Lamphun Provinces, Thailand in 2017. MRSA were screened and identified using mannitol salt agar, biochemical and antimicrobial susceptibility testing, multiplex PCR, and the SCCmec typing. The prevalence of MRSA was 7.9% (16/202) and 19.3% (6/31) among SPP and swine farms. All isolates were multidrug-resistant, and 55 of 59 isolates (93%) contained the type-IX SCCmec element. Data analysis indicated that education, working time, contact frequency, working solely with swine production, and personal hygiene were significantly related to MRSA acquisition (p < 0.05). The multivariate analysis revealed that pig farming experience, working days, and showering were good predictors for MRSA carriage among SPP (area under the curve (AUC) = 0.84). The biosecurity protocols and tetracycline use were significantly associated with MRSA detection in pig farms (p < 0.05). Hence, the active surveillance of MRSA and further development of local/national intervention for MRSA control are essential.

中文翻译：

---

泰国清迈和南奔府养猪生产人员中抗SCCmec IX-耐甲氧西林金黄色葡萄球菌的获得风险因素

在泰国北部的猪和人中发现了带有IX型葡萄球菌盒式染色体mec（SCC mec）的耐甲氧西林金黄色葡萄球菌（MRSA）。但是，需要了解猪生产人员（SPP）中该MRSA株的流行和获得风险因素。2017年从泰国清迈和南奔府的202个自愿性SPP农场和31个猪场收集了鼻拭子样本和数据。使用甘露醇盐琼脂，生化和抗菌药敏试验，多重PCR和SCC mec筛选和鉴定了MRSA。打字。在SPP和养猪场中，MRSA的患病率为7.9％（16/202）和19.3％（6/31）。所有分离株均耐多药，59个分离株中有55个（93％）含有IX型SCC mec元素。数据分析表明，受教育程度，工作时间，接触频率，只从事养猪工作和个人卫生与MRSA的获得密切相关（p <0.05）。多元分析表明，养猪场的工作经验，工作日和洗澡时间是SPP间MRSA携带的良好预测指标（曲线下面积（AUC）= 0.84）。生物安全性方案和四环素的使用与养猪场的MRSA检测显着相关（p<0.05）。因此，积极监测MRSA并进一步发展对MRSA控制的本地/国家干预至关重要。