Abstract Selenium is a nutrient element essential for the growth and reproduction of plants and animals. Generally, selenium nanoparticle has a high level of absorption in regular supplementation comparative to selenium. In this study, selenium nanoparticles have been synthesized using a cell-free extract of Geobacillus as a reducing and stabilizing agent. This is for the first time; Geobacillus has been used for the synthesis of selenium nanoparticles. The cell-free extract of bacteria has active enzymes that are responsible for the reduction of sodium selenite to prepare selenium nanoparticles. The cell-free extract increased the reduction process comparative to the bacterial reduction. The biosynthesized nanoparticles were characterized by UV-visible spectrophotometry, transmission electron microscopy along with selected area electron diffraction, Fourier transform infrared spectroscopy, and energy-dispersive X-ray spectroscopy. The spherical nanoparticles have diameters in the range of 30 to 60 nm. The antioxidant activity was evaluated by various antioxidant assays. This study revealed that biofunctionalized selenium nanoparticle has more antioxidant activity than sodium selenite and cell-free extract. Abbreviations: ABTS: 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid); DPPH: 1,1-diphenyl-2-picrylhydrazyl; EDTA: ethylenediaminetetraacetic acid; EDAX: energy dispersive X-ray analysis; FRAP: ferric reducing antioxidant power; FTIR: Fourier transform infrared spectroscopy; PBS: phosphate buffered saline; PMSF: phenylmethylsulfonyl fluoride; SAED: selected area electron diffraction; SeNP: selenium nanoparticles; TEM: transmission electron microscopy; TPTZ- 2,4,6-Tri(2-pyridyl)-s-triazine; YAG: yttrium aluminium garnet

中文翻译：

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使用土芽孢杆菌的无细胞提取物生物合成的硒纳米颗粒的抗氧化活性

摘要 硒是动植物生长繁殖所必需的营养元素。通常，与硒相比，硒纳米颗粒在定期补充中具有较高的吸收水平。在这项研究中，使用土芽孢杆菌的无细胞提取物作为还原剂和稳定剂合成了硒纳米颗粒。这是第一次；地芽孢杆菌已被用于合成硒纳米颗粒。细菌的无细胞提取物具有活性酶，负责还原亚硒酸钠以制备硒纳米颗粒。与细菌减少相比，无细胞提取物增加了减少过程。生物合成的纳米粒子通过紫外-可见分光光度法表征，透射电子显微镜以及选区电子衍射、傅里叶变换红外光谱和能量色散 X 射线光谱。球形纳米粒子的直径在 30 至 60 nm 范围内。通过各种抗氧化试验评估抗氧化活性。该研究表明，生物功能化硒纳米颗粒比亚硒酸钠和无细胞提取物具有更高的抗氧化活性。缩写：ABTS：2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)；DPPH：1,1-二苯基-2-苦基肼；EDTA：乙二胺四乙酸；EDAX：能量色散X射线分析；FRAP：铁还原抗氧化能力；FTIR：傅里叶变换红外光谱；PBS：磷酸盐缓冲盐水；PMSF：苯甲基磺酰氟；SAED：选区电子衍射；神经网络：硒纳米颗粒；TEM：透射电子显微镜；TPTZ-2,4,6-三(2-吡啶基)-s-三嗪；YAG：钇铝石榴石