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Beneficial Chromosomal Integration of the Genes for CTX-M Extended-Spectrum {beta}-Lactamase in Klebsiella pneumoniae for Stable Propagation
mSystems ( IF 6.4 ) Pub Date : 2020-09-29 , DOI: 10.1128/msystems.00459-20 Eun-Jeong Yoon 1, 2 , Bareum Gwon 1, 2 , Changseung Liu 1, 2 , Dokyun Kim 1, 2 , Dongju Won 1 , Sung Gyun Park 1 , Jong Rak Choi 1 , Seok Hoon Jeong 2, 3
mSystems ( IF 6.4 ) Pub Date : 2020-09-29 , DOI: 10.1128/msystems.00459-20 Eun-Jeong Yoon 1, 2 , Bareum Gwon 1, 2 , Changseung Liu 1, 2 , Dokyun Kim 1, 2 , Dongju Won 1 , Sung Gyun Park 1 , Jong Rak Choi 1 , Seok Hoon Jeong 2, 3
Affiliation
The acquired CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales are of great concern in clinical settings because they limit therapeutic options for patients infected by the pathogens. An intriguing clonality of CTX-M ESBL-producing Klebsiella pneumoniae blood isolates was observed from a national cohort study, and comparative genomics were assessed for the 115 K. pneumoniae blood isolates carrying the blaCTX-M gene. The plasmid preference of particular clones of a sequence type (ST) was assessed by liquid mating. A quarter of the blaCTX-M gene-carrying K. pneumoniae blood isolates harbor the gene in their chromosome, and most of those with the built-in blaCTX-M gene belonged either to ST307 or ST48. Notably, all 16 K. pneumoniae ST48 isolates harbored two copies of the blaCTX-M-15 gene in the chromosome. The chromosomal integration of the blaCTX-M-15 gene was mostly derived from the ISEcp1-targeting 5-bp AT-rich locus in the chromosome. The IS26-mediated chromosomal integration occurred when the upstream ISEcp1 from the blaCTX-M gene was truncated, targeting the anchor IS26 copy in the chromosome. Higher transfer efficiency of the blaCTX-M-15 gene-carrying FIA:R plasmid was observed in ST17 than that of the blaCTX-M-14 gene-carrying FIB:FII plasmid. The transfer efficiency of the plasmid differed by isolate among the ST307 members. The K. pneumoniae clones ST307 and ST48 harboring the blaCTX-M-15 gene in the chromosome were able to disseminate stably in clinical settings regardless of the environmental pressure, and the current population of K. pneumoniae blood isolates was constructed. Further follow-up is needed for the epidemiology of this antimicrobial resistance.
中文翻译：
肺炎克雷伯菌中 CTX-M 超广谱{β}-内酰胺酶基因的有益染色体整合以稳定繁殖
获得性 CTX-M 型产超广谱β-内酰胺酶 (ESBL) 的肠杆菌在临床环境中备受关注，因为它们限制了被病原体感染的患者的治疗选择。从一项国家队列研究中观察到产生 CTX-M ESBL 的肺炎克雷伯菌血液分离株的有趣克隆性，并对 115株携带bla CTX-M基因的肺炎克雷伯菌血液分离株进行了比较基因组学评估。通过液体交配评估特定序列类型 (ST) 克隆的质粒偏好。携带bla CTX-M基因的肺炎克雷伯菌的四分之一血液分离株在其染色体中包含该基因，并且大多数具有内置bla CTX-M基因的基因属于 ST307 或 ST48。值得注意的是，所有 16株肺炎克雷伯菌ST48 分离株的染色体中都含有bla CTX-M-15基因的两个拷贝。bla CTX-M-15基因的染色体整合主要来源于染色体中靶向IS Ecp1 的 5-bp 富含 AT 的基因座。在IS 26当上游IS发生介导的染色体整合ECP1从BLA CTX-M基因被截断，靶向锚26复制到染色体中。在 ST17 中观察到bla CTX-M-15基因携带 FIA:R 质粒的转移效率高于bla CTX-M-14基因携带 FIB:FII 质粒的转移效率。质粒的转移效率因 ST307 成员之间的分离株而异。的肺炎克雷伯菌克隆ST307和ST48窝藏BLA CTX-M-15在染色体基因能够在临床环境中稳定地传播而不管环境压力的，和当前人口肺炎克雷伯菌，构建血液分离物。这种抗菌素耐药性的流行病学需要进一步随访。
更新日期：2020-09-29
中文翻译：
肺炎克雷伯菌中 CTX-M 超广谱{β}-内酰胺酶基因的有益染色体整合以稳定繁殖
获得性 CTX-M 型产超广谱β-内酰胺酶 (ESBL) 的肠杆菌在临床环境中备受关注，因为它们限制了被病原体感染的患者的治疗选择。从一项国家队列研究中观察到产生 CTX-M ESBL 的肺炎克雷伯菌血液分离株的有趣克隆性，并对 115株携带bla CTX-M基因的肺炎克雷伯菌血液分离株进行了比较基因组学评估。通过液体交配评估特定序列类型 (ST) 克隆的质粒偏好。携带bla CTX-M基因的肺炎克雷伯菌的四分之一血液分离株在其染色体中包含该基因，并且大多数具有内置bla CTX-M基因的基因属于 ST307 或 ST48。值得注意的是，所有 16株肺炎克雷伯菌ST48 分离株的染色体中都含有bla CTX-M-15基因的两个拷贝。bla CTX-M-15基因的染色体整合主要来源于染色体中靶向IS Ecp1 的 5-bp 富含 AT 的基因座。在IS 26当上游IS发生介导的染色体整合ECP1从BLA CTX-M基因被截断，靶向锚26复制到染色体中。在 ST17 中观察到bla CTX-M-15基因携带 FIA:R 质粒的转移效率高于bla CTX-M-14基因携带 FIB:FII 质粒的转移效率。质粒的转移效率因 ST307 成员之间的分离株而异。的肺炎克雷伯菌克隆ST307和ST48窝藏BLA CTX-M-15在染色体基因能够在临床环境中稳定地传播而不管环境压力的，和当前人口肺炎克雷伯菌，构建血液分离物。这种抗菌素耐药性的流行病学需要进一步随访。
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