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Acquisition of plasmids conferring carbapenem and aminoglycoside resistance and loss of surface-exposed macromolecule structures as strategies for the adaptation of Acinetobacter baumannii CC104O/CC15P strains to the clinical setting
Microbial Genomics ( IF 3.9 ) Pub Date : 2020-09-01 , DOI: 10.1099/mgen.0.000360
María M Cameranesi 1 , Julian Paganini 1 , Adriana S Limansky 1 , Jorgelina Moran-Barrio 1 , Suzana P Salcedo 2 , Alejandro M Viale 1 , Guillermo D Repizo 1, 2
Affiliation  

Acinetobacter baumannii (Aba) is an emerging opportunistic pathogen associated to nosocomial infections. The rapid increase in multidrug resistance (MDR) among Aba strains underscores the urgency of understanding how this pathogen evolves in the clinical environment. We conducted here a whole-genome sequence comparative analysis of three phylogenetically and epidemiologically related MDR Aba strains from Argentinean hospitals, assigned to the CC104O/CC15P clonal complex. While the Ab244 strain was carbapenem-susceptible, Ab242 and Ab825, isolated after the introduction of carbapenem therapy, displayed resistance to these last resource β-lactams. We found a high chromosomal synteny among the three strains, but significant differences at their accessory genomes. Most importantly, carbapenem resistance in Ab242 and Ab825 was attributed to the acquisition of a Rep_3 family plasmid carrying a bla OXA-58 gene. Other differences involved a genomic island carrying resistance to toxic compounds and a Tn10 element exclusive to Ab244 and Ab825, respectively. Also remarkably, 44 insertion sequences (ISs) were uncovered in Ab825, in contrast with the 14 and 11 detected in Ab242 and Ab244, respectively. Moreover, Ab825 showed a higher killing capacity as compared to the other two strains in the Galleria mellonella infection model. A search for virulence and persistence determinants indicated the loss or IS-mediated interruption of genes encoding many surface-exposed macromolecules in Ab825, suggesting that these events are responsible for its higher relative virulence. The comparative genomic analyses of the CC104O/CC15P strains conducted here revealed the contribution of acquired mobile genetic elements such as ISs and plasmids to the adaptation of A. baumannii to the clinical setting.

中文翻译:

获得赋予碳青霉烯类和氨基糖苷类抗性以及表面暴露大分子结构丢失的质粒作为鲍曼不动杆菌 CC104O/CC15P 菌株适应临床环境的策略

鲍曼不动杆菌 ( Aba ) 是一种与医院感染相关的新兴机会性病原体。Aba菌株多药耐药性 (MDR) 的迅速增加凸显了了解这种病原体如何在临床环境中进化的紧迫性。我们在这里对来自阿根廷医院的三种系统发育和流行病学相关的 MDR Aba菌株进行了全基因组序列比较分析,分配给 CC104 O /CC15 P克隆复合体。虽然 Ab244 菌株对碳青霉烯敏感,但在引入碳青霉烯疗法后分离出的 Ab242 和 Ab825 显示出对这些最后一种资源 β-内酰胺的抗性。我们发现三个菌株之间的染色体同线性很高,但它们的附属基因组存在显着差异。最重要的是,Ab242 和 Ab825 中的碳青霉烯抗性归因于获得了携带bla OXA-58基因的 Rep_3 家族质粒。其他差异涉及对有毒化合物具有抗性的基因组岛和 Tn 10分别为 Ab244 和 Ab825 独有的元素。同样值得注意的是,Ab825 中发现了 44 个插入序列 (IS),而 Ab242 和 Ab244 中分别检测到了 14 个和 11 个。此外,与大蜡螟感染模型中的其他两种菌株相比,Ab825 显示出更高的杀伤能力。对毒力和持久性决定因素的研究表明,编码 Ab825 中许多表面暴露大分子的基因的丢失或 IS 介导的中断,表明这些事件是其较高的相对毒力的原因。在此进行的 CC104 O /CC15 P菌株的比较基因组分析揭示了获得的移动遗传元件如 IS 和质粒对适应 A. baumannii 到临床环境。
更新日期:2020-09-29
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