Breeding for salt tolerance or abiotic stress, in general, requires rapid but reliable screening protocols that reflect the actual field situation as much as possible. A collection of 200 crop wild relative (CWR)‐derived BC₃F_3‐4 rice (Oryza sativa L.) lines developed at the International Rice Research Institute (IRRI) were evaluated by farmers in the Mekong Delta over two seasons for agronomic performance. Fifty stable BC₃F₅ lines were selected and subsequently screened in hydroponics using three NaCl concentrations to assess their phenotypic response to salt stress. The lines and check varieties were grown in a salinized Yoshida nutrient solution at three concentrations: 68, 102, and 13 mM NaCl. Several lines were identified to be tolerant to salinity stress and ANOVA showed significant differences among genotypes and NaCl concentrations. Root and shoot growth parameters showed an inverse relationship with increasing NaCl concentration. Population genetic analysis suggested four groups of genotypes, where the median salt injury score across the three NaCl concentrations was identified as the main clustering factor. Lines from Cluster 3 were identified as the most promising donors of salt tolerance.

中文翻译：

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湄公河三角洲农民选用CWR水稻系对盐胁迫的表型响应

通常，耐盐或非生物胁迫的育种需要快速但可靠的筛选方案，以尽可能反映实际田间情况。湄公河三角洲的农民在两个季节内对国际稻米研究所（IRRI）开发的200株野生野生亲缘稻（CWR）衍生的BC ₃ F _3-4稻（Oryza sativa L.）品系进行了评估。 。五十稳定BC ₃ F ₅选择品系，随后使用三种NaCl浓度在水培法中进行筛选，以评估其对盐胁迫的表型响应。将品系和检查品种在盐碱化的吉田营养液中以三种浓度生长：68、102和13 mM NaCl。鉴定出几个耐盐胁迫的品系，ANOVA显示基因型和NaCl浓度之间存在显着差异。根冠生长参数与NaCl浓度增加呈反比关系。人口遗传分析提出了四组基因型，其中三个NaCl浓度的盐分中值被确定为主要的聚类因子。集群3的品系被确定为最有前途的耐盐性捐助者。