当前位置: X-MOL 学术Lett. Appl. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Detection of Raillietina saudiae from the domestic pigeon in Saudi Arabia through 18S and 28S rDNA genes
Letters in Applied Microbiology ( IF 2.4 ) Pub Date : 2020-10-14 , DOI: 10.1111/lam.13400
S. Al‐Quraishy 1 , R. Abdel‐Gaber 1, 2 , M.A. Dkhil 1, 3 , A.S. Abdel‐Baki 4 , M. Alotaibi 1 , W. Alhafidh 1 , N. Al‐Houshany 1
Affiliation  

Raillietina saudiae is a well‐studied avian gastrointestinal parasite belonging to the family Davaineidae and is the most prevalent cyclophyllid tapeworm infecting pigeon in Saudi Arabia. The present study considered as a complementary analysis of Al‐Quraishy et al. (2019; Parasitol Int 71, 59–72) with molecular studies for two ribosomal DNA genes employed for precise recognition of this Raillietina species. The annotated partial 18S and 28S rDNA gene regions were found to be 888 and 900 bp long that utilized further to elucidate their genetic relationships at species level using maximum likelihood method. The query sequence of R. saudiae is well aligned and placed within the Davaineidae family, with the same clade of all species of Raillietina that well separated from other cyclophyllidean cestodes especially taeniid and hymenolepid species. Sequence data recorded the monophyly of Raillietina species. The current phylogeny supports the usage of the partial 18S and 28S rDNA genes as reliable markers for phylogenetic reconstructions.

中文翻译:

通过18S和28S rDNA基因检测沙特阿拉伯家鸽的Raillietina saudiae

Raillietina saudiae 是一种经过充分研究的鸟类胃肠道寄生虫,属于 Davaineidae 科,是沙特阿拉伯最常见的感染鸽子的环叶绦虫。本研究被认为是对 Al-Quraishy 等人的补充分析。(2019; Parasitol Int 71, 59-72) 对用于精确识别这种 Raillietina 物种的两个核糖体 DNA 基因进行分子研究。发现注释的部分 18S 和 28S rDNA 基因区域长 888 和 900 bp,进一步利用最大似然法在物种水平阐明它们的遗传关系。R. saudiae 的查询序列被很好地对齐并置于 Davaineidae 科内,与所有 Raillietina 物种的相同进化枝与其他 cyclophyllidean 绦虫特别是 taeniid 和 hymenolepid 物种很好地分离。序列数据记录了 Raillietina 物种的单一性。当前的系统发育支持使用部分 18S 和 28S rDNA 基因作为系统发育重建的可靠标记。
更新日期:2020-10-14
down
wechat
bug