Currently, knowledge is limited concerning the impact of a Lactobacillus plantarum JL01 diet for weaned piglets on caecal bacteria and metabolite profiles. In our experiments, 24 weaned piglets were randomly divided into two groups; each piglet in the treatment groups (Cec‐Lac) was fed a basic diet and administered 10 ml of L. plantarum JL01 (1·0 × 109 CFU per ml) every day. The control group (Cec‐Con) was fed a basic diet. After feeding for 28 days, we analysed the parameters of the caecal digesta of weaned piglets. We used 16S rDNA gene sequencing and mass spectrometry (MS)‐based metabolomics techniques to investigate the effect of a L. plantarum JL01 diet on intestinal microbial composition and its metabolite profiles in the caecum contents of weaned piglets. The results showed that the richness estimators (ACE and Chao indices) in the caecal bacteria increased in the Cec‐Lac group. Prevotella_2 and Desulfovibrio decreased significantly, while Pantoea and Rectale_group increased in the caecum of weaned piglets in the Cec‐Lac group. Furthermore, Pearson's correlation analysis revealed that the genus Rectale_group was positively correlated with indole‐3‐acetic acid (P < 0·05), and the genus Pantoea had the same correlation with 1‐palmitoyl lysophosphatidic acid. The metabolomics analysis revealed that the L. plantarum JL01 diet supplementation had significant effects on tryptophan metabolism and fat digestion and absorption. The results indicated that the L. plantarum JL01 dietary supplementation not only altered the microbial composition but also mediated tryptophan metabolism and fat digestion and absorption in the caecum, factors that may further affect the health of the host.

中文翻译：

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饲喂植物乳杆菌JL01日粮对断奶仔猪盲肠细菌和代谢物的影响

目前，关于植物乳杆菌 JL01 日粮对断奶仔猪的盲肠细菌和代谢物谱的影响的知识有限。在我们的实验中，24头断奶仔猪被随机分为两组；治疗组 (Cec-Lac) 中的每头仔猪都喂食基本饮食，并每天给予 10 ml 植物乳杆菌 JL01（1·0 × 109 CFU/ml）。对照组 (Cec-Con) 喂食基本饮食。饲喂 28 天后，我们分析了断奶仔猪盲肠食糜的参数。我们使用 16S rDNA 基因测序和基于质谱 (MS) 的代谢组学技术来研究植物乳杆菌 JL01 饮食对断奶仔猪盲肠内容物中肠道微生物组成及其代谢物谱的影响。结果表明，盲肠细菌的丰富度估计值（ACE 和 Chao 指数）在 Cec-Lac 组中增加。Prevotella_2 和 Desulfovibrio 显着减少，而 Pantoea 和 Rectale_group 在 Cec-Lac 组断奶仔猪盲肠中增加。此外，Pearson 相关分析显示 Rectale_group 属与吲哚-3-乙酸呈正相关（P < 0·05），泛菌属与 1-棕榈酰溶血磷脂酸具有相同的相关性。代谢组学分析表明，植物乳杆菌 JL01 膳食补充剂对色氨酸代谢和脂肪消化吸收有显着影响。结果表明，L.