Abstract Microbial degradation of polyethylene (PE) mulching films has attracted much attention, but to date its relatively rapid biodegradation was limited. Here, a consortium of two bacterial isolates, Acinetobacter sp. strain NyZ450 and Bacillus sp. strain NyZ451 from the gut of PE-feeding Tenebrio molitor larvae (mealworms), was suggested to utilize PE, although each strain had no such ability alone. This consortium removed approximately 18% of PE film after 30 days at 23 °C. High-temperature gel permeation chromatography analysis indicated that the number-, weight-, and size-averaged molecular weights of PE were decreased by 14%, 24% and 21% from their respective initial values of 27,494 Da, 179,374 Da and 697,487 Da after a 20-day incubation with this consortium. Under scanning electron microscope, a biofilm formed by these strains was also observed on the film's surface. Fourier transform infrared spectroscopy analysis indicated that the films incubated with these two strains or either of them were all oxidized compared with the control without inoculation. Plate colony counting and electron microscopic observation showed that strain NyZ450 was predominant in the co-culture. This study enriched microbial resources for PE degradation and provided an example for the interaction of two bacterial strains for enhanced PE degradation.

中文翻译：

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不动杆菌共培养对聚乙烯地膜的生物降解。菌株 NyZ450 和芽孢杆菌。从黄粉虫幼虫中分离的菌株 NyZ451

摘要 聚乙烯（PE）地膜的微生物降解引起了广泛关注，但迄今为止其相对快速的生物降解能力有限。在这里，两个细菌分离物的联合体，不动杆菌属。菌株 NyZ450 和芽孢杆菌。来自喂食 PE 的黄粉虫幼虫（粉虫）肠道的菌株 NyZ451 被建议利用 PE，尽管每个菌株都没有这种能力。该联盟在 23 °C 下 30 天后去除了大约 18% 的 PE 薄膜。高温凝胶渗透色谱分析表明，PE 的数均、重均和尺寸平均分子量分别比初始值 27,494 Da、179,374 Da 和 697,487 Da 降低了 14%、24% 和 21%。与该财团进行为期 20 天的孵化。在扫描电子显微镜下，在薄膜表面也观察到由​​这些菌株形成的生物膜。傅里叶变换红外光谱分析表明，与未接种的对照相比，用这两种菌株或其中一种菌株孵育的薄膜均被氧化。平板菌落计数和电子显微镜观察表明菌株 NyZ450 在共培养中占优势。该研究丰富了PE降解的微生物资源，并为两种菌株相互作用增强PE降解提供了一个例子。平板菌落计数和电子显微镜观察表明菌株 NyZ450 在共培养中占优势。该研究丰富了PE降解的微生物资源，并为两种菌株相互作用增强PE降解提供了一个例子。平板菌落计数和电子显微镜观察表明菌株 NyZ450 在共培养中占优势。该研究丰富了PE降解的微生物资源，并为两种菌株相互作用增强PE降解提供了一个例子。