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Advancing d-amino acid-containing peptide discovery in the metazoan
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ( IF 3.2 ) Pub Date : 2020-09-28 , DOI: 10.1016/j.bbapap.2020.140553
David H Mast 1 , James W Checco 2 , Jonathan V Sweedler 1
Affiliation  

The discovery of enzyme-derived d-amino acid-containing peptides (DAACPs) that have physiological importance in the metazoan challenges previous assumptions about the homochirality of animal proteins while simultaneously revealing new analytical challenges in the structural and functional characterization of peptides. Most known DAACPs have been identified though laborious activity-guided purification studies or by homology to previously identified DAACPs. Peptide characterization experiments are increasingly dominated by high throughput mass spectrometry-based peptidomics, with stereochemistry rarely considered due to the technical challenges of identifying l/d isomerization. This review discusses the prevalence of enzyme-derived DAACPs among animals and the physiological consequences of peptide isomerization. Also highlighted are the analytical methods that have been applied for structural characterization/discovery of DAACPs, including results of several recent studies using non-targeted discovery methods for revealing novel DAACPs, strongly suggesting that more DAACPs remain to be uncovered.



中文翻译:

推进后生动物中含有 d-氨基酸的肽的发现

在后生动物中具有生理重要性的酶衍生的含d-氨基酸的肽 (DAACP) 的发现挑战了先前关于动物蛋白质同手性的假设,同时揭示了肽的结构和功能表征方面的新分析挑战。大多数已知的 DAACP 已通过费力的活动引导纯化研究或通过与先前鉴定的 DAACP 的同源性进行鉴定。肽表征实验越来越多地由基于高通量质谱的肽组学主导,由于识别l / d的技术挑战,很少考虑立体化学异构化。本综述讨论了动物中酶衍生 DAACP 的流行以及肽异构化的生理后果。还强调了已应用于 DAACP 结构表征/发现的分析方法,包括最近几项使用非靶向发现方法揭示新型 DAACP 的研究结果,强烈表明仍有更多 DAACP 有待发现。

更新日期:2020-10-13
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