Within the European Union (EU), edible insects need to be approved as “Novel Food” according to Regulation (EU) 2015/2283 and must comply with the requirements of European food law with regard to microbiological and chemical food safety. Substrates used for feeding insects are susceptible to the growth of Fusarium spp. and consequently to contamination with trichothecene mycotoxins. Therefore, the current study aimed to investigate the influence of T-2 and HT-2 toxins on the larval life cycle of yellow mealworm (Tenebrio molitor (L.)) and to study the transfer of T-2, HT-2, T-2 triol and T-2 tetraol in the larvae. In a 4-week feeding study, T. molitor larvae were kept either on naturally (oat flakes moulded with Fusarium sporotrichioides) or artificially contaminated oat flakes, each at two levels (approximately 100 and 250 μg/kg total T-2 and HT-2). Weight gain and survival rates were monitored, and mycotoxins in the feeding substrates, larvae and residues were determined using LC-MS/MS. Larval development varied between the diets and was 44% higher for larvae fed artificially contaminated diets. However, the artificially contaminated diets had a 16% lower survival rate. No trichothecenes were detected in the surviving larvae after harvest, but T-2 and HT-2 were found both in the dead larvae and in the residues of naturally and artificially contaminated diets.

在欧盟 (EU) 内，根据法规 (EU) 2015/2283，食用昆虫需要被批准为“新型食品”，并且必须符合欧洲食品法关于微生物和化学食品安全的要求。用于喂养昆虫的基质对镰刀菌的生长敏感。并因此受到单端孢霉烯真菌毒素的污染。因此，本研究旨在探讨T-2和HT-2毒素对黄粉虫( Tenebrio molitor (L.))幼虫生命周期的影响，研究T-2、HT-2、T -2 三醇和 T-2 四醇在幼虫中。在一项为期 4 周的饲养研究中，黑粉虱幼虫被自然饲养（用镰刀菌模制的燕麦片) 或人工污染的燕麦片，每种有两个水平（T-2 和 HT-2 总量约为 100 和 250 μg/kg）。监测体重增加和存活率，并使用 LC-MS/MS 测定饲养基质、幼虫和残留物中的霉菌毒素。幼虫的发育因日粮而异，喂食人工污染日粮的幼虫高出 44%。然而，人为污染的饮食的存活率降低了 16%。在收获后的存活幼虫中未检测到单端孢霉烯，但在死亡幼虫和自然和人工污染的饲料残留物中均发现了 T-2 和 HT-2。