The generation of transgenic floricultural crops for flower color modification requires a transgene that enhances anthocyanin accumulation and a flower-specific promoter with stable expression throughout flowering stage. Here, the anthocyanin regulatory R2R3-MYB transcription factor (TF) RsMYB1 was placed under the control of the stable flower-specific promoter (InMYB1) to enhance anthocyanin accumulation in Petunia hybrida cv. Mirage Rose. RsMYB1 overexpression with the flower-specific promoter enhanced anthocyanin accumulation only in floral tissues via the upregulation of key anthocyanin biosynthetic genes. The target gene introgression to another Petunia hybrida cv. White Hawaiian lacking anthocyanins also changed the flower color from white to intense pink in the F₁ hybrids by upregulating the biosynthetic genes. Target gene inheritance in the F₂ hybrids also produced diverse flower colors, redder than those of non-inheritance F₂ hybrids. RsMYB1 overexpression using the flower-specific promoter (InMYB1) enhanced anthocyanin levels in the petals of transgenic Petunia and its related hybrids. Thus, this system can be used as a promising tool for intensifying the flower color of other commercially important floricultural crops. In addition, the obtained F₂MW11 and F₂MW12 lines are potential commercial cultivars for the floriculture and landscape industries due to its attractive flower color pattern.

用于花色修饰的转基因花农作物的产生需要增强花色苷积累的转基因和在整个开花阶段具有稳定表达的花特异性启动子。在这里，将花色苷调节性R2R3-MYB转录因子（TF）RsMYB1置于稳定的花特异性启动子（InMYB1）的控制下，以增强矮牵牛cv中花色苷的积累。幻影玫瑰。具有花特异性启动子的RsMYB1过表达仅通过上调关键花色苷生物合成基因来增强花色苷在花卉组织中的积累。靶基因渗入另一株矮牵牛简历。缺乏花色素苷的白色夏威夷人还通过上调生物合成基因，将F ₁杂种中的花色从白色变为强烈的粉红色。F ₂杂种中的靶基因遗传也产生了多种花色，比非遗传性F ₂杂种更红。使用花特异性启动子（InMYB1）的RsMYB1过表达增强了转基因矮牵牛及其相关杂种的花瓣中的花色苷水平。因此，该系统可用作增强其他商业上重要的花卉作物的花色的有前途的工具。另外，得到的F ₂ MW11和F ₂MW12系由于其诱人的花朵颜色图案而成为花艺和园林业的潜在商业栽培品种。