Two Gram-stain-positive, rod-shaped, endospore-forming bacteria, designated 12200R-189^T and 14171R-81^T were isolated from the rhizosphere of tomato plants. The 16S rRNA gene sequence similarity between strains 12200R-189^T and 14171R-81^T were 97.2%. Both strains showed the highest 16S rRNA gene sequence similarities to Paenibacillus sacheonensis SY01^T (96.3% and 98.0%, respectively). The genome of strain 12200R-189^T was approximately 6.7 Mb in size with 5,750 protein-coding genes (CDSs) and the G + C content was 58.1 mol%, whereas that of strain 14171R-81^T comprised one chromosome of 7.0 Mb and two plasmids (0.2 Mb each) with 6,595 CDSs and the G + C content was 54.5 mol%. Comparative genome analysis revealed that average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among 12200R-189^T, 14171R-81^T, and other closely related species were below the cut-off levels 95% and 70%, respectively. Strain 12200R-189^T grew at a temperature range of 15–40°C, pH 6.0–9.0, and 0–3% NaCl (w/v), whereas strain 14171R-81^T grew at a temperature range of 10–37°C, pH 6.0–8.0, and 0–1% NaCl (w/v). Menaquinone-7 (MK-7) was the only isoprenoid quinone detected in both strains. The predominant cellular fatty acids (> 10%) were iso-C_15:0, anteiso-C_15:0, and iso-C_16:0. The polar lipids of strain 12200R-189^T were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), aminophospholipid (APL), phospholipid (PL), phosphatidylglycolipid (PGL), and four aminophosphoglycolipids (APGLs) and those of strain 14171R-81^T were DPG, PG, PE, APL, three PLs, two PGLs, and three APGLs. Based on phylogenetic, genomic, phenotypic, and chemotaxonomic analyses, strains 12200R-189^T and 14171R-81^T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus lycopersici sp. nov. and Paenibacillus rhizovicinus sp. nov. are proposed. The type strains are 12200R-189^T (= KACC 19916^T = CCTCC AB 2020027^T) and 14171R-81^T (= KACC 19915^T = CCTCC AB 2020026^T).

从番茄植物的根际分离出两种革兰氏染色阳性、棒状、内生孢子形成细菌，命名为 12200R-189 ^T和 14171R-81 ^{T。菌株12200R-189 T}和14171R-81 ^T之间的16S rRNA基因序列相似性为97.2%。两种菌株与Paenibacillus sacheonensis SY01 ^T的 16S rRNA 基因序列相似性最高（分别为 96.3% 和 98.0%）。菌株 12200R-189 ^T的基因组大小约为 6.7 Mb，具有 5,750 个蛋白质编码基因 (CDS)，G + C 含量为 58.1 mol%，而菌株 14171R-81 ^{T的基因组大小约为}包含一个 7.0 Mb 的染色体和两个质粒（每个 0.2 Mb），含有 6,595 个 CDS，G + C 含量为 54.5 mol%。^{比较基因组分析显示，12200R-189 T}、14171R-81 ^T和其他密切相关物种的平均核苷酸同一性 (ANI) 和数字 DNA-DNA 杂交 (dDDH) 值低于 95% 和 70% 的临界值，分别。菌株 12200R-189 ^T在 15–40°C、pH 6.0–9.0 和 0–3% NaCl (w/v) 的温度范围内生长，而菌株 14171R-81 ^T在 10–37° 的温度范围内生长C、pH 6.0–8.0 和 0–1% NaCl (w/v)。Menaquinone-7 (MK-7) 是在两种菌株中检测到的唯一异戊二烯醌。主要的细胞脂肪酸 (> 10%) 是 iso-C _15:0 , anteiso-C_15:0和 iso-C _16:0。菌株12200R-189 ^T的极性脂质为双磷脂酰甘油（DPG）、磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、氨基磷脂（APL）、磷脂（PL）、磷脂酰糖脂（PGL）和四种氨基磷酸糖脂（APGLs）以及菌株的极性脂质。 14171R-81 ^T是 DPG、PG、PE、APL、三个 PL、两个 PGL 和三个 APGL。基于系统发育、基因组、表型和化学分类学分析，菌株 12200R-189 ^T和 14171R-81 ^T代表了类芽孢杆菌属的两个新物种，其名称为Paenibacillus lycopersici sp。十一月 和根际类芽孢杆菌sp。十一月 被提议。典型菌株是 12200R-189 ^T (= KACC 19916 ^T = CCTCC AB 2020027 ^T ) 和 14171R-81 ^T (= KACC 19915 ^T = CCTCC AB 2020026 ^T )。