Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this method has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM method for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.

标记标记诱变（STM）是一种高通量遗传技术，可用于通过构建大量具有独特DNA识别标签的突变菌株，将它们合并并筛选它们的特定表型特征来研究基因的功能。STM首先被设计用于鉴定有助于病原体宿主毒性或感染性的基因。最近，该方法也已用于鉴定具有特定表型的突变体，例如抗真菌药物的耐药性和增殖。在本研究中，我们描述了一种STM方法，该方法使用突变体文库鉴定有助于新型隐球菌感染性的基因，其中每个菌株都标记有独特的DNA序列。