Recently, we introduced magnetophoretic circuits, composed of overlaid magnetic and metallic layers, as a novel single-cell analysis (SCA) tool. We showed the ability of these circuits in organizing large single-particle and particle-pair arrays. Assembling the cells in microarrays is performed with the ultimate goal of running temporal phenotypic analyses. However, for long-term studies, a suitable microenvironment for the cells to normally grow and differentiate is needed. Towards this goal, in this study, we run required biocompatibility tests, based on which we make the magnetophoretic-based microchip a suitable home for the cells to grow. The results confirm the ability of these chips in cell handling and show no unwanted cell behavior alteration due to the applied shear stress on them, the magnetic labeling, or the microenvironment. After this achievement, this tool would be ready for running important single-cell studies in oncology, virology, and medicine.

中文翻译：

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磁泳电路生物相容性

最近，我们引入了由重叠磁性层和金属层组成的磁泳电路，作为一种新型的单细胞分析 (SCA) 工具。我们展示了这些电路在组织大型单粒子和粒子对阵列方面的能力。在微阵列中组装细胞的最终目标是运行时间表型分析。然而，对于长期研究，需要一个适合细胞正常生长和分化的微环境。为了实现这一目标，在这项研究中，我们进行了所需的生物相容性测试，在此基础上，我们使基于磁泳的微芯片成为细胞生长的合适场所。结果证实了这些芯片在细胞处理中的能力，并且没有显示出由于对它们施加的剪切应力、磁标记或微环境而导致的不必要的细胞行为改变。