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Application of two charge transfer complex formation reactions for selective determination of metformin hydrochloride in pharmaceuticals and urine
Future Journal of Pharmaceutical Sciences Pub Date : 2020-09-28 , DOI: 10.1186/s43094-020-00082-6
Nagaraju Rajendraprasad , Kanakapura Basavaiah

Metformin hydrochloride (MFH) is a biguanide class anti-diabetic drug used to treat type-2 diabetes mellitus. Its reaction with two charge-transfer complexing agents, p-chloranilic acid (PCA) and 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) in acetonitrile medium to yield coloured products measurable at wavelengths of maxima 530.0 and 460.0 nm, respectively, was conveniently used to develop two spectrophotometric methods for analyses of bulk sample and tablets. The effect of solvent, reagent concentration and reaction time to form charge-transfer (CT) complexes was meticulously studied and optimized. Under optimised conditions, the absorbance at the respective wavelength of maximum versus concentration of MFH was in linear correlation for the range from 8.0 to 320.0 and from 1.6 to 64.0 μg mL-1 in PCA and DDQ methods, respectively, and correspondingly, the values of molar absorptivity of 0.733 × 103 and 0.257 × 104 L mol-1 cm-1 and Sandell sensitivity of 0.3620 and 0.0644 μg cm-2. The quantification (QL) and detection (DL) limits were 2.67 and 0.88 μg mL-1 for PCA method, and 0.33 and 0.11 μg mL-1 for DDQ method. The new methods were emerged as repeatable and reproducible, with replicate measurements for intra- and inter-day variations as showed by obtained RSD values of < 2%. Within a day and between day relative errors were ≤ 2.18%. Methods were also validated for robustness, ruggedness and selectivity and agreeing results were produced. The methods were used to analyse MFH-containing tablets very accurately and precisely as reflected by the mean recovery value close to 100% and lower RSD values, respectively. Analysis of spiked human urine yielded excellent mean recoveries, indicating the absence of interference from endogenous substances.

中文翻译:

两种电荷转移配合物形成反应在药物和尿液中盐酸二甲双胍选择性测定中的应用

盐酸二甲双胍(MFH)是用于治疗2型糖尿病的双胍类抗糖尿病药物。它与两种电荷转移络合剂,对氯苯甲酸(PCA)和2,3-二氯-5,6-二氰基对苯醌(DDQ)在乙腈介质中反应,生成可在最大530.0和0.52波长测量的有色产物。 460.0 nm分别方便地用于开发两种分光光度法来分析大块样品和片剂。认真研究和优化了溶剂,试剂浓度和反应时间对形成电荷转移(CT)配合物的影响。在最佳条件下,在PCA和DDQ方法中,最大波长相对于MFH浓度的各自波长处的吸光度分别为8.0至320.0和1.6至64.0μgmL-1呈线性相关,摩尔吸光度分别为0.733×103和0.257×104 L mol-1 cm-1,桑德尔灵敏度为0.3620和0.0644μgcm-2。对于PCA方法,定量(QL)和检测(DL)的限度分别为2.67和0.88μgmL-1,对于DDQ方法,其定量限为0.33和0.11μgmL-1。新方法以可重复性和可重复性出现,对日内和日间变化进行了重复测量,如获得的RSD值<2%所示。一天之内和一天之间的相对误差≤2.18%。还验证了方法的鲁棒性,耐用性和选择性,并得出了令人满意的结果。该方法用于非常准确和精确地分析含MFH的片剂,这分别由平均回收率接近100%和较低的RSD值反映出来。
更新日期:2020-09-28
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