SummaryThe aim of the present study was to investigate several common conditions that may potentially be correlated with follicular oxidative status during an intracytoplasmic sperm injection (ICSI) cycle and that include the serum oestrogen level on the day of oocyte pick-up, maternal age and pregnancy outcome. Patients that were enrolled in the study were classified randomly into three groups using their numerical order. The first group were classified based on maternal age (<35 and ≥35 years) (n = 398), the second group on the serum oestradiol (E2) level on the day of human chorionic gonadotropin (hCG) administration (levels >90th percentile and ≤ 90th percentile) (n = 491) and the third group on pregnancy outcome (positive/negative) (n = 376). The groups were matched for the other variables (stimulation protocol, dose of gonadotropin, duration of stimulation, antral follicle count, body mass index, basal follicle stimulating hormone (FSH), and E2 levels and day of hCG trigger) to prevent the possible contribution of those parameters to the results. Each group was matched for other variables (stimulation protocol, dose of gonadotrophin, duration of stimulation, antral follicle count, body mass index, basal FSH and E2 levels and day of hCG trigger) that may have affected the outcome, except for the parameter under investigation. Maternal age (P = 0.044,168 r = 0.418), oestrogen level on day of hCG administration (P = 0.001, r = 0.436) and pregnancy outcome (AUC = 0.65, P = 0.071) were found to be correlated with follicular oxidative status. The results obtained will help us to shield patients from possible situations that may cause oxidative stress and therefore adverse outcomes of an ICSI cycle.

中文翻译：

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胞浆内精子注射周期中卵泡氧化应激的相关因素及其对颗粒细胞的影响

摘要本研究的目的是调查可能与胞浆内精子注射 (ICSI) 周期中卵泡氧化状态相关的几种常见情况，包括取卵当天的血清雌激素水平、母亲年龄和怀孕。结果。参加研究的患者按照数字顺序随机分为三组。第一组根据产妇年龄（<35 岁和≥35 岁）进行分类（n= 398)，第二组在人绒毛膜促性腺激素 (hCG) 给药当天血清雌二醇 (E2) 水平（水平 > 90% 和 ≤ 90%）（n= 491）和第三组妊娠结局（阳性/阴性）（n= 376)。各组匹配其他变量（刺激方案、促性腺激素剂量、刺激持续时间、窦卵泡计数、体重指数、基础促卵泡激素 (FSH) 以及 E2 水平和 hCG 触发天数），以防止可能的贡献这些参数的结果。每组都匹配了可能影响结果的其他变量（刺激方案、促性腺激素剂量、刺激持续时间、窦卵泡计数、体重指数、基础 FSH 和 E2 水平以及触发 hCG 的天数），除了下面的参数调查。产妇年龄（磷= 0.044,168r= 0.418)，hCG 给药当天的雌激素水平 (磷= 0.001,r= 0.436) 和妊娠结局 (AUC = 0.65,磷= 0.071）被发现与卵泡氧化状态相关。获得的结果将帮助我们保护患者免受可能导致氧化应激和 ICSI 周期不良结果的可能情况的影响。