NO₃-N and dye colors discharged from textile wastewater pose environmental problems in Thailand. This study aimed to observe the nitrogen removal rate (NRR) with and without RB-5 color contamination via hydrogenotrophic denitrification (HD) processing, which uses H₂ gas as electron donor to reduce NO₃-N and NO₂-N; comparing with bioreactors treatment to evaluate systems that can simultaneously remove NO₃-N and dye color. Five reactors under different operation and gas supply conditions were set-up under HRT of 24 h, including an aerobic reactor using air, two anaerobic reactors using argon and H_2, and a combined process using intermittent air/argon and air/H₂. NRR without dye varied between 45 and 90% for H₂ and air/H₂ by HD processing, while it was completely removed when adding color. H₂ and air/H₂ reactors experienced partial decolorization of approximately 20–30%, whereas the other three reactors remained unchanged. Effluent of NO₃-N were close to wastewater standards, but the color was still easy to detect, which indicated that the treatment time needs to be sufficient. In conclusion, HD and intermittent air/H₂ processing can completely remove NO₃-N and NO₂-N when contaminated with RB-5 color. Furthermore, RB-5 did not affect the NRR, whereas some particles of dye color can also reduce in these processes.

从纺织废水中排出的NO ₃ -N和染料颜色在泰国造成了环境问题。本研究旨在通过氢营养反硝化（HD）工艺观察带有和不带有RB-5颜色污染的脱氮率（NRR），该工艺使用H ₂气体作为电子供体来还原NO ₃ -N和NO ₂ -N。与生物反应器处理进行比较，以评估可同时去除NO ₃ -N和染料颜色的系统。在24小时HRT下设置了五个在不同运行和气体供应条件下的反应器，包括使用空气的好氧反应器，使用氩气和H _2的两个厌氧反应器以及使用间歇性空气/氩气和空气/ H ₂的组合过程。通过HD处理，无染料的NRR对于H ₂和空气/ H ₂在45％到90％之间变化，而在添加颜色时被完全去除。H ₂和空气/ H ₂反应器的部分脱色率约为20–30％，而其他三个反应器保持不变。NO ₃ -N的出水接近废水标准，但颜色仍易于检测，表明处理时间需要足够。总之，HD和间歇性的空气/ H ₂处理可以完全去除NO ₃ -N和NO ₂-N被RB-5颜色污染时。此外，RB-5不会影响NRR，而在这些过程中某些染料颜色的颗粒也会减少。