Cell migration is a dynamic process that entails extensive protein synthesis and recycling, structural remodeling, and considerable bioenergetic demand. Autophagy is one of the pathways that maintain cellular homeostasis. Time-lapse imaging of autophagosomes and ATP/ADP levels in migrating cells in the rostral migratory stream of mouse revealed that decreases in ATP levels force cells into the stationary phase and induce autophagy. Pharmacological or genetic impairments of autophagy in neuroblasts using either bafilomycin, inducible conditional mice, or CRISPR/Cas9 gene editing decreased cell migration due to the longer duration of the stationary phase. Autophagy is modulated in response to migration-promoting and inhibiting molecular cues and is required for the recycling of focal adhesions. Our results show that autophagy and energy consumption act in concert in migrating cells to dynamically regulate the pace and periodicity of the migratory and stationary phases to sustain neuronal migration.

中文翻译：

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ATP/ADP 水平与自噬之间的动态相互作用维持体内神经元迁移

细胞迁移是一个动态过程，需要大量的蛋白质合成和回收、结构重塑和大量的生物能量需求。自噬是维持细胞稳态的途径之一。小鼠吻端迁移流中迁移细胞中自噬体和 ATP/ADP 水平的延时成像显示，ATP 水平的降低迫使细胞进入静止期并诱导自噬。使用巴弗洛霉素、诱导性条件小鼠或 CRISPR/Cas9 基因编辑的神经母细胞自噬的药理学或遗传损伤会由于静止期的持续时间较长而减少细胞迁移。自噬被调节以响应迁移促进和抑制分子线索，并且是粘着斑再循环所必需的。