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Direct translation of climbing fiber burst-mediated sensory coding into post-synaptic Purkinje cell dendritic calcium
eLife ( IF 7.7 ) Pub Date : 2020-09-28 , DOI: 10.7554/elife.61593
Seung-Eon Roh 1, 2, 3, 4, 5 , Seung Ha Kim 1, 2 , Changhyeon Ryu 1, 2, 3 , Chang-Eop Kim 1, 6 , Yong Gyu Kim 1, 2, 3 , Paul F Worley 5 , Sun Kwang Kim 4 , Sang Jeong Kim 1, 2, 3
Affiliation  

Climbing fibers (CFs) generate complex spikes (CS) and Ca2+ transients in cerebellar Purkinje cells (PCs), serving as instructive signals. The so-called 'all-or-none' character of CSs has been questioned since the CF burst was described. Although recent studies have indicated a sensory-driven enhancement of PC Ca2+ signals, how CF responds to sensory events and contributes to PC dendritic Ca2+ and CS remains unexplored. Here, single or simultaneous Ca2+ imaging of CFs and PCs in awake mice revealed the presynaptic CF Ca2+ amplitude encoded the sensory input’s strength and directly influenced post-synaptic PC dendritic Ca2+ amplitude. The sensory-driven variability in CF Ca2+ amplitude depended on the number of spikes in the CF burst. Finally, the spike number of the CF burst determined the PC Ca2+ influx and CS properties. These results reveal the direct translation of sensory information-coding CF inputs into PC Ca2+, suggesting the sophisticated role of CFs as error signals.

中文翻译:

攀缘纤维爆发介导的感觉编码直接翻译成突触后浦肯野细胞树突钙

攀爬纤维 (CF) 在小脑浦肯野细胞 (PC) 中产生复杂的尖峰 (CS) 和 Ca2+ 瞬变,作为指导信号。自从描述了 CF 突发以来,CS 的所谓“全有或全无”特性就受到了质疑。尽管最近的研究表明 PC Ca2+ 信号的感官驱动增强,但 CF 如何响应感官事件并有助于 PC 树突状 Ca2+ 和 CS 仍未探索。在这里,清醒小鼠中 CF 和 PC 的单次或同时 Ca2+ 成像显示突触前 CF Ca2+ 幅度编码感觉输入的强度,并直接影响突触后 PC 树突状 Ca2+ 幅度。CF Ca2+ 振幅的感官驱动变异性取决于 CF 爆发中的尖峰数量。最后,CF 爆发的尖峰数决定了 PC Ca2+ 流入和 CS 特性。
更新日期:2020-09-28
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