Mammalian female meiosis must be tightly regulated to produce high‐quality mature oocytes for subsequent regular fertilization and healthy live birth of the next generation. GTPases control many important signal pathways involved in diverse cellular activities. ADP‐ribosylation factor family members (Arfs) in mice possess GTPase activities, and some members have been found to function in meiosis. However, whether other Arfs play a role in meiosis is unknown. In this study, we found that Arl2 and Arf5 are the richest among Arfs in mouse oocytes, and they are more abundant in oocytes than in granular cells. Furthermore, Arl2 and Arf5 depletion both impeded meiotic progression, but by affecting spindles and microfilaments, respectively. Moreover, Arl2 and Arf5 depletion both significantly increased regular reactive oxygen species levels and decreased mitochondrial membrane potential and autophagy, indicating that oocyte quality was damaged by Arl2 and Arf5 depletion. These results suggest that Arl2 and Arf5 are two novel essential GTPases required for oocyte meiosis and quality control.

中文翻译：

---

GTPases Arf5 和 Arl2 在卵母细胞减数分裂期间部分功能明显。

哺乳动物雌性减数分裂必须受到严格调控，以产生高质量的成熟卵母细胞，以便随后正常受精和下一代的健康活产。GTPases 控制着许多重要的信号通路，这些信号通路涉及不同的细胞活动。小鼠中的 ADP-核糖基化因子家族成员 (Arfs) 具有 GTPase 活性，并且已经发现一些成员在减数分裂中起作用。然而，其他 Arfs 是否在减数分裂中发挥作用尚不清楚。在这项研究中，我们发现 Arl2 和 Arf5 是小鼠卵母细胞中 Arfs 中最丰富的，并且它们在卵母细胞中比颗粒细胞中更丰富。此外，Arl2 和 Arf5 消耗都阻碍了减数分裂进程，但分别影响纺锤体和微丝。而且，Arl2 和 Arf5 消耗均显着增加常规活性氧水平并降低线粒体膜电位和自噬，表明 Arl2 和 Arf5 消耗损害了卵母细胞质量。这些结果表明 Arl2 和 Arf5 是卵母细胞减数分裂和质量控制所需的两种新型必需 GTP 酶。