The medicinal plant Catharanthus roseus produces numerous secondary metabolites of interest for the treatment of many diseases – most notably for the terpene indole alkaloid (TIA) vinblastine, which is used in the treatment of leukemia and Hodgkin's lymphoma. Historically, methyl jasmonate (MeJA) has been used to induce TIA production, but in the past, this has only been investigated in whole seedlings, cell culture, or hairy root culture. This study examines the effects of the phytohormones MeJA and ethylene on the induction of TIA biosynthesis and accumulation in the shoots and roots of 8‐day‐old seedlings of two varieties of C. roseus. Using LCMS and RT‐qPCR, we demonstrate the importance of variety selection, as we observe markedly different induction patterns of important TIA precursor compounds. Additionally, both phytohormone choice and concentration have significant effects on TIA biosynthesis. Finally, our study suggests that several early‐induction pathway steps as well as pathway‐specific genes are likely to be transcriptionally regulated. Our findings highlight the need for a complete set of'omics resources in commonly used C. roseus varieties and the need for caution when extrapolating results from one cultivar to another.

中文翻译：

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代谢组学分析揭示了植物品种和激素处理方法的选择可调节长春花中长春花生物碱的产生

药用植物长春花（Catharanthus roseus）产生许多感兴趣的次级代谢产物，可用于治疗多种疾病，其中最显着的是用于吲哚生物碱（TIA）长春碱，用于治疗白血病和霍奇金淋巴瘤。从历史上看，茉莉酸甲酯（MeJA）已用于诱导TIA产生，但在过去，仅在整苗，细胞培养或毛根培养中进行了研究。这项研究探讨了植物激素MeJA和乙烯对两种变种玫瑰花C.roseus的8日龄幼苗的TIA生物合成和芽和根中积累的影响。。使用LCMS和RT-qPCR，我们证明了品种选择的重要性，因为我们观察到了重要的TIA前体化合物明显不同的诱导模式。此外，植物激素的选择和浓度对TIA生物合成都有重要影响。最后，我们的研究表明，几个早期诱导途径步骤以及特定于途径的基因可能受到转录调控。我们的研究结果突显了一套完整的of'omics常用资源的需求长春花品种和外推从一个品种到另一个结果时需要谨慎。