The current study investigated the immunomodulating and osteoblast differentiation potential of the natural compounds from Leea macrophylla (LMN). Immunomodulatory effects have been investigated by the phagocytosis of Candida albicans using polymorphonuclear neutrophil cells in the in vitro slide method. A bioactivity‐guided fractionation technique was used to evaluate the stimulating effect of L. macrophylla methanol extract on osteoblast differentiation using mouse osteoblastic cells. A low dose of LMN was found to stimulate the phagocytic effect better than a higher dose. The natural compounds from L. macrophylla have significantly increased alkaline phosphatase (ALP) and osteocalcin activities. The LMN promoted the osteoblast differentiation through upregulation of ALP, osteocalcin, and type 1 collagen in a dose‐dependent manner. These natural compounds also upregulated ALP, osteocalcin, and type 1 collagen gene expressions. The data suggest that LMN has potential anabolic sequel on bone formation and osteoblast differentiation.

中文翻译：

---

大叶李的天然化合物通过碱性磷酸酶、1 型胶原蛋白和骨钙素基因表达增强吞噬作用并促进成骨细胞分化

目前的研究调查了大叶李(LMN) 中天然化合物的免疫调节和成骨细胞分化潜力。在体外玻片法中，通过使用多形核中性粒细胞吞噬白色念珠菌，研究了免疫调节作用。使用生物活性引导的分级分离技术评估大叶大叶甲醇提取物对小鼠成骨细胞成骨细胞分化的刺激作用。发现低剂量的 LMN 比高剂量更能刺激吞噬作用。L. macrophylla的天然化合物碱性磷酸酶（ALP）和骨钙素活性显着增加。LMN 通过以剂量依赖性方式上调 ALP、骨钙素和 1 型胶原蛋白来促进成骨细胞分化。这些天然化合物还上调了 ALP、骨钙素和 1 型胶原基因的表达。数据表明LMN 对骨形成和成骨细胞分化具有潜在的合成代谢作用。