Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder in women of reproductive age. Some studies have investigated metabolic alterations in plasma and follicular fluid from PCOS patients, but they did not control for obesity or insulin resistance (IR); additionally, correlation analysis of metabolites is sparse. Accordingly, in this study, we aimed to examine metabolic differences owing to the pathogenesis of PCOS, identify the hub metabolites and investigate its associations with androgens. We applied GC–MS platform coupled with a correlation network approach to analyze follicular fluid samples from 32 PCOS patients without obesity and IR and 31 healthy women. Thirty significantly altered metabolites in PCOS patients were enriched in amino acid metabolism. l‐Phenylalanine, l‐tryptophan, pyroglutamic acid, l‐tyrosine, l‐leucine and l‐valine were screened as hub metabolites in metabolic correlation network. Among them, increased l‐tryptophan and l‐tyrosine were altered hub metabolites, and they had a more significant impact on the metabolic change of PCOS. In addition, l‐tryptophan and l‐tyrosine were significantly positively associated with serum androgens levels in the PCOS. Our results suggest that disorders of amino acid metabolism, especially tryptophan and tyrosine metabolism, might play an important role in the development of PCOS in predisposed women without obesity and IR.

中文翻译：

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卵泡液的代谢组学和相关网络分析揭示了色氨酸，左旋酪氨酸和多囊卵巢综合征之间的关联

多囊卵巢综合征（PCOS）是育龄妇女的一种内分泌和代谢疾病。一些研究调查了PCOS患者血浆和卵泡液中的代谢变化，但它们并不能控制肥胖或胰岛素抵抗（IR）。另外，代谢物的相关性分析很少。因此，在这项研究中，我们旨在检查由于PCOS的发病机理引起的代谢差异，鉴定中枢代谢产物并研究其与雄激素的关系。我们将GC–MS平台与相关网络方法结合使用，分析了32例无肥胖和IR的PCOS患者和31例健康女性的卵泡液样本。在PCOS患者中，三十种显着改变的代谢产物富含氨基酸代谢。升苯丙氨酸，升色氨酸，焦谷氨酸，升-酪氨酸，升-亮氨酸和升-缬氨酸筛选作为代谢相关性网络集线器代谢物。其中，增加的l-色氨酸和l-酪氨酸改变了中枢代谢产物，它们对PCOS的代谢变化具有更大的影响。此外，升-色氨酸和升-酪氨酸被显著与血清雄激素相关的PCOS水平。我们的结果表明，在没有肥胖和IR的易感女性中，氨基酸代谢异常，尤其是色氨酸和酪氨酸代谢异常可能在PCOS的发生中起重要作用。