LncRNA growth arrest special 5 (GAS5) and microRNA-106b (miR-106b) have been reported to be involved in the regulation of gliomas. However, their precise mechanisms in regulating the progression and development of gliomas remain unclear. We aimed to investigate the interaction between GAS5 and miR-106b, and their influence on the proliferation, migration, and invasion of gliomas cells. Western blotting and qRT-PCR were applied for measuring expression of protein and mRNA, respectively. The proliferation, migration, and invasion of cells were measured by MTT, wound healing, and transwell assays, respectively. Dual luciferase reporter assay was applied for confirming the binding site between miR-106b and GAS5, miR-106b and PTEN. Significant higher expression of miR-106b, and lower expression of GAS5 and PTEN in the glioma tissues were observed. The binding sites between GAS5 and miR-106b, miR-106b and PTEN were identified. GAS5 could regulate the expression of PTEN through targeting miR-106b, and further influence EMT process, and the proliferation, migration, and invasion of gliomas cells. Meanwhile, PTEN could remarkably inhibited the proliferation, migration and invasion of glioma cells. The influence of PTEN on glioma cells and EMT was similar to GAS5. GAS5 could regulate the EMT process, and the migration of gliomas cells through miR-106b targeting PTEN. Therefore, our findings may provide a new thought for the study of pathogenesis and treatment of glioma.

据报道，LncRNA 生长停滞特殊 5 (GAS5) 和 microRNA-106b (miR-106b) 参与了胶质瘤的调节。然而，它们在调节胶质瘤进展和发展方面的确切机制仍不清楚。我们旨在研究 GAS5 和 miR-106b 之间的相互作用，以及它们对胶质瘤细胞增殖、迁移和侵袭的影响。Western印迹和qRT-PCR分别用于测量蛋白质和mRNA的表达。分别通过 MTT、伤口愈合和 transwell 测定法测量细胞的增殖、迁移和侵袭。应用双荧光素酶报告基因测定来确认 miR-106b 和 GAS5、miR-106b 和 PTEN 之间的结合位点。观察到胶质瘤组织中 miR-106b 的表达显着升高，而 GAS5 和 PTEN 的表达降低。鉴定了 GAS5 与 miR-106b、miR-106b 和 PTEN 之间的结合位点。GAS5可通过靶向miR-106b调控PTEN的表达，进而影响EMT过程，以及胶质瘤细胞的增殖、迁移和侵袭。同时，PTEN可以显着抑制胶质瘤细胞的增殖、迁移和侵袭。PTEN对胶质瘤细胞和EMT的影响与GAS5相似。GAS5 可以通过靶向 PTEN 的 miR-106b 调节 EMT 过程和胶质瘤细胞的迁移。因此，我们的研究结果可能为胶质瘤的发病机制和治疗研究提供新的思路。和胶质瘤细胞的侵袭。同时，PTEN可以显着抑制胶质瘤细胞的增殖、迁移和侵袭。PTEN对胶质瘤细胞和EMT的影响与GAS5相似。GAS5 可以通过靶向 PTEN 的 miR-106b 调节 EMT 过程和胶质瘤细胞的迁移。因此，我们的研究结果可能为胶质瘤的发病机制和治疗研究提供新的思路。和胶质瘤细胞的侵袭。同时，PTEN可以显着抑制胶质瘤细胞的增殖、迁移和侵袭。PTEN对胶质瘤细胞和EMT的影响与GAS5相似。GAS5 可以通过靶向 PTEN 的 miR-106b 调节 EMT 过程和胶质瘤细胞的迁移。因此，我们的研究结果可能为胶质瘤的发病机制和治疗研究提供新的思路。